BA24 Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1)

BA24
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, Eq, H, Porcine, R, Sh M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      BA24-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~450 kDa (non-reducing conditions) and the ~180 kDa (reducing conditions) thrombospondin protein, in endothelial cells, fibroblasts, platelets, monocytes, macrophages, and tonsil tissue. Does not cross-react with fibronectin, fibrinogen, or von Willebrand factor.
          Catalogue NumberBA24
          Brand Family Calbiochem®
          SynonymsAnti-TSP-1, Anti-TSP
          Application Data
          Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

          Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
          Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
          Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
          Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
          Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
          Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
          Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
          Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
          Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
          Jaffe, E.A., et al. 1983. PNAS 80, 998.
          Saglio, S. and Slayter, H. 1982. Blood 59, 162.
          Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
          Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
          PreservativeNone
          Applications
          Application ReferencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.
          Key Applications Immunofluorescence
          Not Paraffin Sections
          Neutralization Studies
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesElectron Microscopy (see comments)
          Neutralization Studies (see comments and application references)
          Flow Cytometry (see comments)
          Frozen Sections (1:50-1:100)
          Immunoblotting (1:200-1:400)
          Immunofluorescence (see comments)
          Paraffin Sections (not recommended)
          Application CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenreduced and alkylated human thrombospondin
          ImmunogenHuman
          CloneA6.1
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Bovine
          • Canine
          • Horse
          • Human
          • Porcine
          • Rat
          • Sheep
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) Certificates of Analysis

          TitleLot Number
          BA24

          References

          Reference overview
          Dawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
          Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
          Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
          Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
          Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
          Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
          Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
          Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
          Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
          Jaffe, E.A., et al. 1983. PNAS 80, 998.
          Saglio, S. and Slayter, H. 1982. Blood 59, 162.
          Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
          Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-February-2009 JSW
          SynonymsAnti-TSP-1, Anti-TSP
          ApplicationElectron Microscopy (see comments)
          Neutralization Studies (see comments and application references)
          Flow Cytometry (see comments)
          Frozen Sections (1:50-1:100)
          Immunoblotting (1:200-1:400)
          Immunofluorescence (see comments)
          Paraffin Sections (not recommended)
          Application Data
          Detection of human thombospondin by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1:50). Detection: fluorescence.

          Detection of human Thrombospondin by immunoblotting. Sample: Human thrombospondin. Primary antibody: Anti-Thrombospondin (Ab-3) Mouse mAb (A6.1) (Cat. No. BA24) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/Ag14 mouse myeloma cells. Recognizes the ~450 kDa (non-reduced) or the ~180 kDa (reduced) thrombospondin (TSP) protein.
          BackgroundThrombospondin (TSP) is a ~450 kDa glycoprotein synthesized by platelets, as well as by monocytes, macrophages, fibroblasts and endothelial cells. Although TSP is the major α-granule protein of human platelets, the concentration of the protein in circulating plasma is quite low. Upon stimulation of platelets, the TSP is expressed on the cell surface where it plays a role in platelet aggregation and other cell-cell interactions. Studies show that the platelet-monocyte association has been demonstrated to be mediated by the interaction of TSP on the platelet surface with a monocyte TSP receptor. The receptor has been identified immunologically as glycoprotein IV, also known as GPIIIb and CD36, a ~90 kDa membrane glycoprotein expressed on platelets, endothelial cells, erythrocytes, monocytes, and some human tumor cells lines but not on lymphocytes or granulocytes. Recent studies have shown that TSP-1 is a naturally occurring inhibitor of angiogenesis whose actions are mediated by CD36, found on microvascular endothelial cells.
          HostMouse
          Immunogen speciesHuman
          Immunogenreduced and alkylated human thrombospondin
          CloneA6.1
          IsotypeIgG₁
          Speciesbovine, canine, horse, human, porcine, rat, sheep
          Positive controlEndothelial cells, fibroblasts, platelets, monocytes, or macrophages in tonsil tissue
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsDoes not cross-react with fibronectin, fibrinogen, or von Willebrand factor. This antibody will inhibit TSP-collagen interactions. Its binding to TSP is unaffected by glycosaminoglycans (e.g., hyaluronic acid, chrondroitin sulfate and heparin). EDTA (i.e., low concentrations of Ca2+) enhances the binding capacity of Clone A6.1 to TSP. This antibody has been reported to work in immunofluorescence, flow cytometry, and electron microscopy. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesDawson, D.W., et al. 1997. J. Cell Biol. 138, 707.
          Alessio, M., et al. 1996. J. Biol. Chem. 271, 1770.
          Silverstein, R.L., et al. 1989. J. Clin. Invest. 84, 546.
          Clemetson, K.J. and McGregor et al., 1987. In Platelets in Biology and Pathology III, MacIntyre, E.E. and Gordon, J.L., eds. Academic Press, Inc.
          Silverstein, R.L. and Nachman, R.L. 1987. J. Clin. Invest. 79, 867.
          Dixit, V.M., et al. 1986. J. Biol. Chem. 261, 1962.
          Silverstein, R.L., et al. 1986. Arteriosclerosis 6, 245.
          Dixit, V.M., et al. 1985. Biochemistry 24, 4270.
          Leung, L.L.K. 1984. J. Clin. Invest. 74, 1764.
          Jaffe, E.A., et al. 1983. PNAS 80, 998.
          Saglio, S. and Slayter, H. 1982. Blood 59, 162.
          Hagen, I. 1975. Biochem. Biophys. Acta. 392, 242.
          Phillips, D.R. and Agin, P.P. 1977. J. Biol. Chem. 252, 2121.
          Application referencesNeutralization Studies Galvin, N.J., et al. 1985. J. Cell Biol. 101, 1434.