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MABN471 | Anti-Tau Antibody, PAD, clone TNT-1

MABN471
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC, DB, IF, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN471
      ReplacesMAB10417
      DescriptionAnti-Tau Antibody, PAD, clone TNT-1
      Alternate Names
      • Microtubule-associated protein tau
      • Neurofibrillary tangle protein
      • Paired helical filament-tau
      • PHF-tau
      • Tau
      Background InformationTau or Microtubule-associated protein tau (MAPT), also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), is a cytosolic protein that promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of axonal polarity in neurons. Tau binds to and is thought to function as a linker protein between axonal microtubules and neural plasma membrane components. There are multiple isoforms, and the short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. PAD is the phosphatase activating domain, and has been demonstrated to be involved in the inhibition of anterograde, kinesin-based fast axonal transport (FAT) by activating axonal protein phosphatase 1 (PP1) and glycogen synthase kinase 3 (GSK3), independent of microtubule binding. Defects in Tau are thought to be the cause of a number of neurodegenerative diseases, including frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND), Pick disease of the brain (PIDB), corticobasal degeneration (CBD), supranuclear palsy type 1 (PSNP1), Alzheimer disease, and Parkinson disease.
      References
      Product Information
      FormatPurified
      Control
      • rat cortex tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Tau, PAD, clone TNT-1 detects levels of Tau proteins & has been published & validated for use in WB, IHC, IF & ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Dot Blot
      • Immunofluorescence
      • Enzyme Immunoassay (ELISA)
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Tau, PAD in 10 µg of human and mouse cortex tissue lysates.

      Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Tau, PAD in human Alzheimer's brain tissue.

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Tau, PAD in normal and Alzheimer's tissues from entorhinal cortex, hippocampus, inferior temporal gyrus, and superior gyrus of control (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).

      ELISA Analysis: A representative lot from an independent laboratory detected Tau, PAD in recombinant Tau protein (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Tau.
      CloneTNT-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      • MAPTL
      • MTBT1
      • TAU
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~55 kDa observed. Many isoforms are known to exist between 35-80 kDa. An uncharacterized band may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat cortex tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Tau, PAD in 10 µg of rat cortex tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tau, PAD, clone TNT-1 - Q2138464 Q2138464
      Anti-Tau, PAD, clone TNT-1 -2514253 2514253
      Anti-Tau, PAD, clone TNT-1 -2618526 2618526
      Anti-Tau, PAD, clone TNT-1 -2756668 2756668

      References

      Reference overviewPub Med ID
      Pathogenic forms of tau inhibit kinesin-dependent axonal transport through a mechanism involving activation of axonal phosphotransferases.
      Kanaan, Nicholas M, et al.
      J. Neurosci., 31: 9858-68 (2011) 2011

      Show Abstract
      21734277 21734277

      Newsletters / Publications

      Title
      Research Focus - Volume 2, 2013