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MABF249 | Anti-Tapasin Antibody, clone 7F6

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R FC R Purified Monoclonal Antibody
      Catalogue NumberMABF249
      DescriptionAnti-Tapasin Antibody, clone 7F6
      Alternate Names
      • Tapasin
      • TPN
      • TSPN
      • NGS-17
      • TAP-associated protein
      • TAP-binding protein
      Background InformationTapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.
      Product Information
      • HeLa cell lysate
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

      Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.

      Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).
      Biological Information
      ImmunogenLinear peptide corresponding to human Tapasin.
      EpitopeLumenal domain
      Cloneclone 7F6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the Lumenal domain of Tapasin.
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TAPBP
      • NGS17
      • TAPA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~48 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tapasin, clone 7F6 - 2291691 2291691
      Anti-Tapasin, clone 7F6 - Q2201620 Q2201620


      Reference overviewPub Med ID
      Direct evidence that the N-terminal extensions of the TAP complex act as autonomous interaction scaffolds for the assembly of the MHC I peptide-loading complex.
      Hulpke, Sabine, et al.
      Cell. Mol. Life Sci., 69: 3317-27 (2012) 2012

      Show Abstract
      22638925 22638925
      Specific targeting of the EBV lytic phase protein BNLF2a to the transporter associated with antigen processing results in impairment of HLA class I-restricted antigen presentation.
      Horst, Daniëlle, et al.
      J. Immunol., 182: 2313-24 (2009) 2009

      Show Abstract
      19201886 19201886
      A CD8+ T cell immune evasion protein specific to Epstein-Barr virus and its close relatives in Old World primates.
      Hislop, Andrew D, et al.
      J. Exp. Med., 204: 1863-73 (2007) 2007

      Show Abstract
      17620360 17620360
      The first N-terminal transmembrane helix of each subunit of the antigenic peptide transporter TAP is essential for independent tapasin binding.
      Koch, Joachim, et al.
      FEBS Lett., 580: 4091-6 (2006) 2006

      Show Abstract
      16828748 16828748