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MABS410 | Anti-TPX2 Antibody, clone 18D5

MABS410
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IF, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABS410
      DescriptionAnti-TPX2 Antibody, clone 18D5
      Alternate Names
      • Differentially expressed in cancerous and non-cancerous lung cells 2
      • DIL-2
      • Hepatocellular carcinoma-associated antigen 519
      • Protein fls353
      • Restricted expression proliferation-associated protein 100
      • p100
      • Targeting protein for Xklp2
      • TPX2
      Background InformationTargeting protein for Xklp2 (TPX2), also known as Differentially expressed in cancerous and non-cancerous lung cells 2 (DIL-2) or Restricted expression proliferation-associated protein 100 (p100), is a major spindle assembly factor belonging to the TPX2 family of proteins. Tpx2 is a required component in the normal assembly of mitotic spindles and, during apoptosis, for microtubules. It is also necessary to the nucleation of microtubules in a chromatin and/or kinetechore dependent manner. Tpx2 activates Aurora-A kinase through promotion of autophosphorylation, protects the activated kinase from dephosphorylation, and regulates its localization to spindle microtubules. Normal expression of Tpx2 is exclusive to proliferating cells, specifically those transitioning from G1/S to the termination of cytokinesis. Tissue expression has only been observed in lung cancer cell lines. TPX2 interacts with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, -tubulin, and nucleolin among others.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
      Applications
      ApplicationDetect Tpx2 using this mouse monoclonal antibody, Anti-TPX2 Antibody, clone 18D5 validated for use in western blotting, Immunofluorescence & IHC.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunofluorescence Analysis: 1-4 μg/mL from a representative lot detected TPX2 in HeLa cells.

      Immunohistochemistry Analysis: A representative lot detected TPX2 in Immunohistochemistry.
      Biological Information
      ImmunogenAmino Acid: 1-220 of human TPX2
      Clone18D5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TPX2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~97 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MOLT-4 nuclear extract.

      Western Blotting Analysis: 1 μg/mL of this antibody detected TPX2 in MOLT-4 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies