ST1099 Anti-TORC2 (454-607) Rabbit pAb

ST1099
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1099-50UL
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          Glass bottle 50 ul
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          Description
          OverviewRecognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.
          Catalogue NumberST1099
          Brand Family Calbiochem®
          SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
          Application Data
          Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
          References
          ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:2000, see comments)
          Immunoprecipitation (1:100, see comments)
          Application CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TORC2 (454-607) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-TORC2 (454-607) Rabbit pAb Certificates of Analysis

          TitleLot Number
          ST1099

          References

          Reference overview
          Screaton, R.A., et al. 2004. Cell 119, 61.

          Brochure

          Title
          Calbiochem Biologics 32.2
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2007 RFH
          SynonymsAnti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
          ApplicationImmunoblotting (1:2000, see comments)
          Immunoprecipitation (1:100, see comments)
          Application Data
          Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.
          BackgroundTORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
          HostRabbit
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
          FormLiquid
          FormulationUndiluted serum.
          PreservativeNone
          CommentsThis antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFor long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesScreaton, R.A., et al. 2004. Cell 119, 61.