ST1704 Anti-TOMM22 Mouse mAb (4G4)

ST1704
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1704-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.
          Catalogue NumberST1704
          Brand Family Calbiochem®
          SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
          Application Data
          Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

          Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

          Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
          Clone4G4
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TOMM22 Mouse mAb (4G4) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-November-2010 RFH
          SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
          ApplicationELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

          Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

          Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.
          BackgroundThe protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.
          HostMouse
          ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
          Clone4G4
          IsotypeIgG2a
          Specieshuman, mouse, rat
          Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
          Negative control293T cells
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review