ST1705 Anti-TOMM20 Mouse mAb (4F3)

ST1705
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1705-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.
          Catalogue NumberST1705
          Brand Family Calbiochem®
          SynonymsAnti-Translocase of Outer Mitochondrial Membrane 20
          Application Data
          Detection of human TOMM20 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of rat TOMM20 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TOMM20 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TOMM20 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (10 µg/ml). Detection: fluorescence.

          Detection of human TOMM20 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (3 µg/ml). Detection: colorimetric.
          References
          Product Information
          Formliquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlHeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
          Clone4F3
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TOMM20 Mouse mAb (4F3) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-November-2010 JLN
          SynonymsAnti-Translocase of Outer Mitochondrial Membrane 20
          ApplicationELISA (see coments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human TOMM20 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of rat TOMM20 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse TOMM20 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human TOMM20 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (10 µg/ml). Detection: fluorescence.

          Detection of human TOMM20 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM20 Mouse mAb (4F3) (Cat. No. ST1705) (3 µg/ml). Detection: colorimetric.
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.
          BackgroundThe TOMM20 protein is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 , it functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
          HostMouse
          ImmunogenFull-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
          Clone4F3
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlHeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
          Negative control293T cells
          Formliquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review