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AP1159 | Anti-TNFRSF14 Mouse mAb (2G6-2C7)

AP1159
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      AP1159-100UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          100 μg
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          Description
          OverviewRecognizes the TNFRSF14 protein in human pancreatic cancer tissue.
          Catalogue NumberAP1159
          Brand Family Calbiochem®
          SynonymsAnti-Tumor Necrosis Factor Receptor Super family 14
          Application Data
          Detection of human TNFRSF14 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNFRSF14 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1-5 µg/ml). Detection: Chemiluminescence.

          Detection of human TNFRSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human pancreatic cancer tissue. Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1 µg/ml). Detection: coloritmetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlHuman pancreatic cancer tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg)
          Paraffin Sections (1 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human TNFRSF14 (aa 38-248), expressed as a GST fusion protein
          Clone2G6-2C7
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2010 RFH
          SynonymsAnti-Tumor Necrosis Factor Receptor Super family 14
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg)
          Paraffin Sections (1 µg/ml)
          Application Data
          Detection of human TNFRSF14 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with full-length, recombinant, human TNFRSF14 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1-5 µg/ml). Detection: Chemiluminescence.

          Detection of human TNFRSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human pancreatic cancer tissue. Primary antibody: Anti-TNFRSF14 Mouse mAb (2G6-2C7) (Cat. No. AP1159) (1 µg/ml). Detection: coloritmetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the TNFRSF14 protein.
          BackgroundTNFRSF14 is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.
          HostMouse
          ImmunogenFull-length, recombinant, human TNFRSF14 (aa 38-248), expressed as a GST fusion protein
          Clone2G6-2C7
          IsotypeIgG₁
          Specieshuman
          Positive controlHuman pancreatic cancer tissue
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review