DR1041 Anti-TIP60 Rabbit pAb

DR1041
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DR1041-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~60 kDa TIP60 protein in Saos-2 cells.
          Catalogue NumberDR1041
          Brand Family Calbiochem®
          SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
          Application Data
          Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
          References
          ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
          Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
          Tang, Y., et al. 2006. Mol. Cell 24, 827.
          Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive controlSaos-2 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:3000)
          Immunoprecipitation (1:100)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant, human TIP60 protein
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TIP60 Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-TIP60 Rabbit pAb Certificates of Analysis

          TitleLot Number
          DR1041

          References

          Reference overview
          Murr, R., et al. 2006. Nat. Cell Biol. 8, 91.
          Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
          Tang, Y., et al. 2006. Mol. Cell 24, 827.
          Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision23-August-2007 RFH
          SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
          ApplicationImmunoblotting (1:3000)
          Immunoprecipitation (1:100)
          Application Data
          Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~60 kDa TIP60 protein.
          BackgroundTIP60 (accession no. Q92993) is a 60 kDa protein that is a member of the MYST family, which is a distinct class of histone acetyl-transferases (HAT). TIP60 was initially identified as a protein that interacts with the HIV protein, Tat, and shares no homology with the HATs, CBP/p300, or PCAF. TIP60 was recently shown to modulate DNA repair and p53 function. TIP60 also directly acetylates γ-H2AX and, together with the cofactor, TRRAP, binds to the chromatin surrounding sites of DNA double-stranded breaks in vivo. Furthermore, TIP60 associates with MYC and recruits it to the chromatin, which results in MYC-induced actetylation of histone H4. TIP60 directly associates with p53 and specifically acetylates it at K120, modulating the decision between cell cycle arrest and apoptosis. TIP60 acts as a transcription coactivator of p53 that enhances the activation of p21 and puma, leading to cell growth arrest and apoptosis. K120 actetylation is essential for p53-mediated puma activation and apoptosis, but is dispensable for both p53-mediated growth arrest, through p21 activation, and p53/MDM2 interaction. K120 is not acetylated by p300 and its actetylation by TIP60 does not affect the stability or DNA binding activity of p53.
          HostRabbit
          Immunogenrecombinant, human TIP60 protein
          IsotypeIgG
          Specieshuman
          Positive controlSaos-2 cells
          FormLiquid
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
          Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
          Tang, Y., et al. 2006. Mol. Cell 24, 827.
          Frank, S.R., et al. 2003. EMBO Rep. 6, 575.