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AB6000 | Anti-TIMP-3 Antibody, CT

AB6000
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Po, Ca, Mk, Pm, Eq, B WB, ICC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB6000
      ReplacesAB802
      DescriptionAnti-TIMP-3 Antibody, CT
      Alternate Names
      • MIG-5
      • TIMP metallopeptidase inhibitor 3
      • Tissue inhibitor of metalloproteinases 3
      Background InformationTissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HL-60 cell lysate in PMA-conditioned media.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.
      Species Reactivity
      • Human
      • Rat
      • Pig
      • Canine
      • Monkey
      • Primate
      • Horse
      • Bovine
      Species Reactivity NoteHuman and rat. Predicted to react with pig, canine, monkey, primate, equine, and bovine based on 100% sequence homology and mouse at 95% homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq]
      Gene Symbol
      • TIMP3
      • K222
      • TIMP-3
      • SFD
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. May form part of a tissue- specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

      SIZE: 211 amino acids; 24145 Da

      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.

      DISEASE: Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.

      SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
      Molecular WeightBands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TIMP-3, C-terminus 2470959
      Anti-TIMP-3, C-terminus - 2145977 2145977
      Anti-TIMP-3, C-terminus - 2370189 2370189
      Anti-TIMP-3, C-terminus - NG1756936 NG1756936
      Anti-TIMP-3, C-terminus - 1983711 1983711
      Anti-TIMP-3, C-terminus - 2023685 2023685
      Anti-TIMP-3, C-terminus - 2246836 2246836
      Anti-TIMP-3, C-terminus - NG1710337 NG1710337
      Anti-TIMP-3, C-terminus - NG1824720 NG1824720
      Anti-TIMP-3, C-terminus - NG1862872 NG1862872
      Anti-TIMP-3, C-terminus - NRG1671155 NRG1671155
      Anti-TIMP-3, C-terminus -2604556 2604556
      Anti-TIMP-3, C-terminus -2676631 2676631
      Anti-TIMP-3, C-terminus -2716341 2716341
      Anti-TIMP-3, C-terminus -2727260 2727260
      Anti-TIMP-3, C-terminus -2798585 2798585

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies