IM32 Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)

IM32
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, R M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM32-100UG
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      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes.
          Catalogue NumberIM32
          Brand Family Calbiochem®
          SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
          Application Data
          Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesFujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
          Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J. F., 1991. FASEB J. 5, 2145.
          Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
          Product Information
          DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
          FormLiquid
          FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
          Positive controlrTIMP-1 Protein (Cat. Nos. PF019 or PF020)
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Paraffin Sections
          Application NotesImmunoblotting (1 µg/ml, see application references)
          Immunocytochemistry (see application references)
          Paraffin Sections (25 µg/ml, see application references, see comments)
          Application CommentsAlso recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified, bovine dental pulp TIMP-1
          ImmunogenBovine
          Clone7-6C1
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Bovine
          • Human
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) Certificates of Analysis

          TitleLot Number
          IM32

          References

          Reference overview
          Fujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
          Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J. F., 1991. FASEB J. 5, 2145.
          Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-August-2007 RFH
          SynonymsAnti-Tissue Inhibitor of Metalloproteinase-1
          ApplicationImmunoblotting (1 µg/ml, see application references)
          Immunocytochemistry (see application references)
          Paraffin Sections (25 µg/ml, see application references, see comments)
          Application Data
          Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the ~28 kDa TIMP-1 protein.
          BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 (also called EPA, Fibroblast collagenase inhibitor or collagenase inhibitor) is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
          HostMouse
          Immunogen speciesBovine
          Immunogenpurified, bovine dental pulp TIMP-1
          Clone7-6C1
          IsotypeIgG₁
          Speciesbovine, human, not mouse, rat
          Positive controlrTIMP-1 Protein (Cat. Nos. PF019 or PF020)
          FormLiquid
          FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAlso recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesFujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237.
          Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
          Woessner, J. F., 1991. FASEB J. 5, 2145.
          Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
          Application referencesImmunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.