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MABE521 | Anti-TIMELESS Antibody, clone EPR5275, Rabbit Monoclonal

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IHC Rb Unpurified Monoclonal Antibody
      Catalogue NumberMABE521
      DescriptionAnti-TIMELESS Antibody, clone EPR5275, Rabbit Monoclonal
      Alternate Names
      • Protein timeless homolog
      • hTIM
      Background InformationProtein timeless homolog (hTIM) and encoded by the gene name TIMELESS or TIM, or TIM1, or TIMELESS1, is required for normal progression of S-phase, and is involved in the circadian rhythm autoregulatory loop. TIMELESS is involved in cell survival after DNA damage or replication stress. TIMELESS is localized in the nucleus and regulated by cell cycle. It is expressed at high levels in S, G2 and M phases, and low levels in G0 and G1 phases.
      Product Information
      PresentationRabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.
      ApplicationThis Anti-TIMELESS Antibody, clone EPR5275, Rabbit Monoclonal is validated for use in Western Blotting and Immunohistochemistry for the detection of TIMELESS.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected TIMELESS in human colon tissue.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C-terminus of human TIMELESS.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TIMELESS
      • TIM
      • TIM1
      • TIMELESS1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~150 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, Jurkat, JAR and BxPC-3 cell lysates.

      Western Blotting Analysis: A 1:50,000-200,000 dilution of this antibody detected TIMELESS in HeLa, Jurkat, JAR and BxPC-3 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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