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MABF931 | Anti-TCR β (mouse) Antibody, clone H57-597

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC, ACT, IP AHm Purified Monoclonal Antibody
      Catalogue NumberMABF931
      DescriptionAnti-TCR β (mouse) Antibody, clone H57-597
      Alternate Names
      • TCR β (mouse)
      Background InformationThe T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The C domain lies proximal to the plasma membrane, while the V domain engages the antigen/MHC complex. Similar to immunoglobulin light chain and heavy chain, the TCR alpha and beta genes (Gene ID 21473 and 21577, respectively, in murine species) undergo VJ and VDJ recombination to generate TCR diversity for recognizing a wide range of antigens. the alpha and beta chains have very short cytoplamic tail and reply on the CD3 (cluster of differentiation 3) co-receptor for transducing signaling further intracellularly.
      Product Information
      PresentationPurified armenian hamster monoclonal IgG antibody in PBS without preservatives.
      ApplicationAnti-TCR β (mouse) Antibody, clone H57-597 is an antibody against TCR β for use in Flow Cytometry, Activity Assay, Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Activity Assay
      • Immunoprecipitation
      Application NotesFlow Cytometry Analysis: A representative lot detected αβ TCR surface expression on 99% of adult lymph node T-cells from mouse strain C3H/HeJ, while only 10% of adult and 1% of newborn thymocyte population were found to be αβ TCR-expressing mature thymocytes (Finkel, T.H., et al. (1989). J. Immunol. 142(9):3006-3012).
      Activity Assay: Representative lots, when immunobilized on well surface, activated murine T cell hybridomas (DO-11.10, 2QK34.7, and A1.1) by cross-linking cell surface αβ TCR. Clone H57-597 did not stimulate T cell hybridomas that express γδ TCR (Shi, Y.F., et al. (1991). J. Immunol. 146(10):3340-3346; (Grégoire, C., et al. (1991). Proc. Natl. Acad. Sci. U. S. A. 88(18):8077-8081; Kubo, R.T., et al. (1989). J. Immunol. 142(8):2736-2742).
      Activity Assay: A representative lot induced intracellular calcium mobilization by cross-linking surface αβ TCR on adult lymph nodes T cells from mouse strain C3H/HeJ (Finkel, T.H., et al. (1989). J. Immunol. 142(9):3006-3012).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated soluble TCR β, but not TCR α chimeric construct (Grégoire, C., et al. (1991). Proc. Natl. Acad. Sci. U. S. A. 88(18):8077-8081).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated αβ TCR from adult lymph nodes T cells and day 19 fetal thymocytes of mouse strain C3H/HeJ (Kubo, R.T., et al. (1989). J. Immunol. 142(8):2736-2742).
      Biological Information
      ImmunogenPurified TCR from mouse T cells.
      EpitopeExtracellular C domain of TCR beta chain
      ConcentrationPlease refer to lot specific datasheet.
      HostArmenian Hamster
      SpecificityClone H57-597 targets the C region of TCR beta chain. Clone H57-597 reacts with alpha/beta TCR, but not with gamma/delta TCR, nor with CD3 (Kubo, R.T., et al. (1989). J. Immunol. 142(8):2736-2742).
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightN/A
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

      Flow Cytometry Analysis: 0.1 µg of this antibody detected TCR β in mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-TCR β (mouse), clone H57-597 -Q2627008 Q2627008


      Reference overviewPub Med ID
      Engineered secreted T-cell receptor alpha beta heterodimers.
      Grégoire, C; Rebaï, N; Schweisguth, F; Necker, A; Mazza, G; Auphan, N; Millward, A; Schmitt-Verhulst, AM; Malissen, B
      Proceedings of the National Academy of Sciences of the United States of America 88 8077-81 1991

      Show Abstract
      1716770 1716770
      In vivo administration of monoclonal antibodies to the CD3 T cell receptor complex induces cell death (apoptosis) in immature thymocytes.
      Shi, YF; Bissonnette, RP; Parfrey, N; Szalay, M; Kubo, RT; Green, DR
      Journal of immunology (Baltimore, Md. : 1950) 146 3340-6 1991

      Show Abstract
      1827483 1827483
      Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.
      Kubo, RT; Born, W; Kappler, JW; Marrack, P; Pigeon, M
      Journal of immunology (Baltimore, Md. : 1950) 142 2736-42 1989

      Show Abstract
      2467936 2467936
      Alpha beta T cell receptor and CD3 transduce different signals in immature T cells. Implications for selection and tolerance.
      Finkel, TH; Marrack, P; Kappler, JW; Kubo, RT; Cambier, JC
      Journal of immunology (Baltimore, Md. : 1950) 142 3006-12 1989

      Show Abstract
      2523422 2523422