A part of MilliporeSigma

ABN1379 | Anti-TAG-1 Antibody

ABN1379
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB, ICC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABN1379
      DescriptionAnti-TAG-1 Antibody
      Alternate Names
      • Contactin-2
      • TAG-1
      • Axonal glycoprotein TAG-1
      • Axonin-1
      • Transient axonal glycoprotein 1
      • TAX-1
      Background InformationContactin-2 (UniProt P22063; also known as Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1) is encoded by the Cntn2 (also known as Tax1) gene (Gene ID 25356) in rat species. Proper localization of the voltage-gated Na+ channels to the node of Ranvier and voltage-gated K+ channels to the juxtaparanode (JXP) along myelinated axons is essential for the fast propagation of action potentials. Localization of clustered ion channels in specific membrane domains depends on specific cell adhesion molecules (CAMs), as well as on the integrity of neighboring domains. Contactin-2/TAG-1 and Caspr2 form a membrane scaffold complex that functions to cluster Kv1 potassium channels at the JXP. Deletion of either of these CAMs causes Kv1 channels to disappear from the JXP and to redistribute along the internode. Contactin-2/TAG-1 is initially produced with an N-terminal signal peptide (a.a. 1-30) and a C-terminal propeptide (a.a. 1016-1040) sequence, the removal of which yields the mature GPI-anchored membrane glycoprotein.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Applications
      ApplicationAnti-TAG-1 Antibody is an antibody against TAG-1 for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: Representative lots detected TAG-1 immunoreactivity at the juxtaparanodal (JXP) region of teased sciatic nerves from wild-type, but not Caspr−/−, Caspr2−/−, or Caspr−/−Caspr2−/− double knockout, mice. Reexpressing full-length Caspr2, but not 4.1-binding sequence (d4.1) deletion Caspr2 mutant, in Caspr2−/− transgenic mice restored JXP TAG-1 immunoreactivity (Gordon, A., et al. (2014). J. Neurosci. 34(45):14820 –14826; Horresh, I., et al. (2010). J. Neurosci. 30(7):2480–2489).
      Immunocytochemistry Analysis: A representative lot detected a loss of TAG-1 immunoreactivity at the juxtaparanodal (JXP) region of teased sciatic nerves isolated from 4.1B−/− mice lacking the cytoskeletal adapter protein 4.1B (Horresh, I., et al. (2010). J. Neurosci. 30(7):2480–2489).
      Biological Information
      ImmunogenFc-tagged recombinant protein corresponding to the Fibronectin type-III domains 1-4 of rat TAG-1.
      EpitopeFibronectin type-III domains 1-4.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cntn2
      • Tax1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~140 kDa observed. Target band appears larger than the calculated molecular weight of 107.3 kDa due to glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected TAG-1 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TAG-1 -Q2638542 Q2638542

      References

      Reference overviewPub Med ID
      Caspr and caspr2 are required for both radial and longitudinal organization of myelinated axons.
      Gordon, A; Adamsky, K; Vainshtein, A; Frechter, S; Dupree, JL; Rosenbluth, J; Peles, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 14820-6 2014

      Show Abstract
      25378149 25378149
      Organization of myelinated axons by Caspr and Caspr2 requires the cytoskeletal adapter protein 4.1B.
      Horresh, I; Bar, V; Kissil, JL; Peles, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience 30 2480-9 2010

      Show Abstract
      20164332 20164332

      Brochure

      Title
      Neuroscience Solutions for productive research