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ABE42 | Anti-TAF(II)250 Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Eq, Ca WB Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABE42
      DescriptionAnti-TAF(II)250 Antibody
      Alternate Names
      • Transcription initiation factor TFIID subunit 1
      • Cell cycle gene 1 protein
      • TBP-associated factor 250 kDa
      • p250
      • Transcription initiation factor TFIID 250 kDa subunit
      • TAF(II)250
      • TAFII-250
      • TAFII250
      Background InformationTAF(II)250, also referred to as TAF1, is an N-terminal kinase and the largest subunit of the human transcription factor TFIID. TAF(II)250 includes serine/threonine kinase domain that has the ability to transphosphorylate and autophosphorylate the basal factor TFIIF large subunit. TAF(II)250 has been shown to form a complex with B-Myb which activated Myb responsive promoters and plays a role in cell cycle regulation. TAF(II)250 is also important in the preservation of Mdm2 phosphorylation and function, which negatively regulates the p53 tumor suppressor protein.
      Product Information
      FormatAffinity Purified
      • HepG2 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-TAF(II)250 Antibody (rabbit polyclonal antibody) validated in WB to detect TAF(II)250 also known as Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TAF(II)250 at the protein kinase 2 domain.
      EpitopeProtein kinase 2 domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes TAF(II)250 at the protein kinase 2 domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Horse
      • Canine
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Equine, and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.[provided by RefSeq].
      Gene Symbol
      • TAF1
      • BA2R
      • CCG1
      • CCGS
      • TAF2A
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium.

      ENZYME REGULATION: Autophosphorylates on Ser residues. Inhibited by retinoblastoma tumor suppressor protein, RB1.

      SUBUNIT STRUCTURE: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen.


      PTM: Phosphorylated by casein kinase II in vitro.

      INVOLVEMENT IN DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.

      SEQUENCE SIMILARITIES: Belongs to the TAF1 family.

      Contains 2 bromo domains.

      Contains 1 HMG box DNA-binding domain.

      Contains 2 protein kinase domains.
      Molecular Weight~ 225 kDa observed. Uncharacterized bands may be observed between ~70 and 220 kDa in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected TAF(II)250 on 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies