574778 Anti-Synapsin I Rabbit pAb

574778
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      574778-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the synapsin I protein in rat brain.
          Catalogue Number574778
          Brand Family Calbiochem®
          References
          ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
          Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
          Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
          Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
          Product Information
          FormLiquid
          FormulationUndiluted serum.
          Positive controlRat brain
          PreservativeNone
          Applications
          Key Applications Immunocytochemistry
          Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:2500-1:10,000)
          Frozen Sections (1:5000)
          Immunoblotting (1:200, chemiluminescence)
          Immunocytochemistry (1:500-1:2500)
          Immunoprecipitation (5 µl/200 µg total protein, see comments)
          Application CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenpurified, bovine brain synapsin I
          ImmunogenBovine
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Synapsin I Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Synapsin I Rabbit pAb Certificates of Analysis

          TitleLot Number
          574778

          References

          Reference overview
          Stone, L.M., et al. 1994. J. Neurosci. 14, 301.
          Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
          Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
          Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-July-2007 RFH
          ApplicationELISA (1:2500-1:10,000)
          Frozen Sections (1:5000)
          Immunoblotting (1:200, chemiluminescence)
          Immunocytochemistry (1:500-1:2500)
          Immunoprecipitation (5 µl/200 µg total protein, see comments)
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the synapsin I protein.
          BackgroundSynapsin I is a neuron specific protein that serves as an excellent marker for synaptic terminals. Can be used to estimate synaptic density and synaptogenesis.
          HostRabbit
          Immunogen speciesBovine
          Immunogenpurified, bovine brain synapsin I
          IsotypeIgG
          Speciesbovine, human, mouse, rat
          Positive controlRat brain
          FormLiquid
          FormulationUndiluted serum.
          PreservativeNone
          CommentsExhibits strong reactivity with the synapsin I doublet by immunoblotting. Immunolabeling is blocked by preadsorption of the antibody with synapsin I. 5 µl will quantitatively immunoprecipitate all synapsin in 200 µg total protein from rat brain. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesStone, L.M., et al. 1994. J. Neurosci. 14, 301.
          Mandell, J.W., et al. 1992. J. Neurosci. 12, 1736.
          Fletcher, T.L., et al. 1991. J. Neurosci. 11, 1617.
          Moore, R.Y., and Berstein, M. 1989. J. Neurosci. 9, 2151.