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MABN1573 | Anti-Synapsin-2 Antibody, clone 19.4

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M WB, IH(P), IP, EA M Purified Monoclonal Antibody
      Catalogue NumberMABN1573
      DescriptionAnti-Synapsin-2 Antibody, clone 19.4
      Alternate Names
      • Synapsin II
      • Synapsin-2
      Background InformationSynapsin-2 (UniProt Q63537; also known as Synapsin II) is encoded by the Syn2 gene (Gene ID 29179) in rat species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.
      Product Information
      PresentationPurified mouse monoclonal IgG2aκ antibody in PBS without preservatives.
      ApplicationAnti-Synapsin-2 Antibody, clone 19.4 is an antibody against Synapsin-2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Enzyme Assay.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Enzyme Assay
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synapsin-2 in human cerebral cortex, mouse cerebellum, and rat cerebral cortex tissue.
      Western Blotting Analysis: Target detection by a representative lot was blocked by a preincubation with phage particles displaying peptides containing the SYIxN consensus sequence (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
      Western Blotting Analysis: A representative lot detected synapsin IIa/IIb, but not Ia/Ib, in rat brain cytosolic extracts (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin IIa/IIb, but not Ia/Ib, from rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
      Enzyme Assay Analysis: A representative lot, when added 2 hours prior to cAMP, prevented PKA-catalyzed phosphorylation of synapsin IIa/IIb in an in vitro kinase assay using rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
      Biological Information
      ImmunogenPurified recombinant rat synapsin-2.
      EpitopeDomains A&B
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 19.4 reacts with both synapsin-2 spliced isoforms (IIa and IIb). Clone 19.4 does not react with any of the two synapsin-1 spliced isoforms (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Syn2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~63 and 52 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-2 in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-Synapsin-2, clone 19.4 -Q2626412 Q2626412


      Reference overviewPub Med ID
      Anti-synapsin monoclonal antibodies: epitope mapping and inhibitory effects on phosphorylation and Grb2 binding.
      Vaccaro, P; Dente, L; Onofri, F; Zucconi, A; Martinelli, S; Valtorta, F; Greengard, P; Cesareni, G; Benfenati, F
      Brain research. Molecular brain research 52 1-16 1997

      Show Abstract
      9450672 9450672


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