Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||ELISA, WB||Gt||Purified||Polyclonal Antibody|
|Description||Anti-Surfactant Protein A Antibody|
|Presentation||Purified immunoglobulin. Liquid in PBS. Contains no preservative.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.|
|Material Size||100 µL|
|GOAT ANTI-HUMAN SURFACTANT PROTEIN A (SP-A) POLYCLONAL ANTIBODY - 2273928||2273928|
|Reference overview||Application||Pub Med ID|
|Pneumoproteins in sewage workers exposed to sewage dust. |
Heldal, KK; Barregard, L; Larsson, P; Ellingsen, DG
International archives of occupational and environmental health 86 65-70 2013
The association between exposure to bacteria and endotoxins in sewage dust and the serum concentrations of pneumoproteins in sewage treatment plant workers were studied.Forty-four workers from eight sewage treatment plants and 38 reference workers participated in the study. Microbial aerosol was collected by personal inhalable samplers. The concentrations of bacteria and endotoxins were determined by fluorescence microscopy and the Limulus assay, respectively. Pneumoproteins (Clara cell protein: CC16, and Surfactant proteins A and D: SP-A, SP-D) were determined by ELISA in blood samples collected post-shift.The exposure to dust ranged from 0.02 to 9.3 (geometric mean (GM) 0.3 mg/m(3), of bacteria from 0.3 to 4,900 × 10(3) (GM 27 × 10(3)) cells/m(3) and endotoxins from 1 to 3,160 (GM 28) EU/m(3). The exposed workers had lower CC16 [arithmetic mean (AM) 4.9 ng/ml] compared to the referents (AM 6.4 ng/ml, p less than 0.01). No significant difference was observed for SP-D and SP-A. Exposure to bacteria was positively associated with CC16 (p less than 0.05) and SP-D (p less than 0.05), adjusting for possible confounders.This study showed that exposed workers had lower serum concentration of CC16 as compared to the referents, which may reflect a long-term effect on secretion of these pneumoproteins. The positive association between exposure to bacteria and the serum concentrations of CC16 and SP-D may be explained by a transient increased permeability of the lung-blood barrier.
|Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome. |
Gregory, T J, et al.
J. Clin. Invest., 88: 1976-81 (1991) 1991
Acute Respiratory Distress Syndrome (ARDS) is characterized by lung injury and damage to the alveolar type II cells. This study sought to determine if endogenous surfactant is altered in ARDS. Bronchoalveolar lavage was performed in patients at-risk to develop ARDS (AR, n = 20), with ARDS (A, n = 66) and in normal subjects (N, n = 29). The crude surfactant pellet was analyzed for total phospholipids (PL), individual phospholipids, SP-A, SP-B, and minimum surface tension (STmin). PL was decreased in both AR and A (3.48 +/- 0.61 and 2.47 +/- 0.40 mumol/ml, respectively) compared to N (7.99 +/- 0.60 mumol/ml). Phosphatidylcholine was decreased in A (62.64 +/- 2.20% PL) compared to N (76.27 +/- 2.05% PL). Phosphatidylglycerol was 11.58 +/- 1.21% PL in N and was decreased to 6.48 +/- 1.43% PL in A. SP-A was 123.64 +/- 20.66 micrograms/ml in N and was decreased to 49.28 +/- 21.68 micrograms/ml in AR and to 29.88 +/- 8.49 micrograms/ml in A. SP-B was 1.28 +/- 0.33 micrograms/ml in N and was decreased to 0.57 +/- 0.24 micrograms/ml in A. STmin was increased in AR (15.1 +/- 2.53 dyn/cm) and A (29.04 +/- 2.05 dyn/cm) compared to N (7.44 +/- 1.61 dyn/cm). These data demonstrate that the chemical composition and functional activity of surfactant is altered in ARDS. Several of these alterations also occur in AR, suggesting that these abnormalities occur early in the disease process.
|Anti-Surfactant Protein A - Data Sheet|