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ABN1003 | Anti-Stearoyl-CoA desaturase 1 Antibody

ABN1003
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB, IHC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABN1003
      DescriptionAnti-Stearoyl-CoA desaturase 1 Antibody
      Alternate Names
      • Stearoyl-CoA desaturase 1
      • Acyl-CoA desaturase 1
      • Delta(9)-desaturase 1
      • Delta-9 desaturase 1
      • Fatty acid desaturase 1
      Background InformationFatty acid desaturases (FADS) catalyze the formation of carbon/carbon double bonds in fatty acids. Stearoyl-CoA desaturase 1 (Scd1), in particular, is an FADS that catalyzes the synthesis of oleic acid by introducing a double bond between C9 and C10 from the carboxyl end of stearic acid. Scd1 activity is essential for the earliest step of autophagosome formation, whose membrane has a high content of unsaturated fatty acids. Scd1 is reported to regulate inflammatory gene expression in adipocytes by influencing DNA methylation at the CpG promoter sites, Scd1 overexpression or silencing results in global DNA hypomethylation or hypermethylation, respectively, in 3T3 adipocytes. Stearoyl-CoA desaturase 1 is a novel therapeutic target in various malignancies, including breast cancer. (PMID 25130896, 25023287, and 25013434). Cat. No. ABN1003, Anti-Stearoyl-CoA desaturase 1, is a purified rabbit polyclonal raised against murine Scd1 (UniProt P13516 & Q547C4) C-terminal sequence that is well conserved in rat (UniProt P07308), but not in human.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS without preservatives.
      Applications
      ApplicationAnti-Stearoyl-CoA desaturase 1 Antibody is an antibody against Stearoyl-CoA desaturase 1 for use in Immunohistochemistry and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Stearoyl-CoA desaturase 1 in microsomal membranes from C57BL/6J mice (Thomas Gettys, Palmetto Biotech).
      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Stearoyl-CoA desaturase 1 in brown and white adipose mouse hepatic tissue sections (Thomas Gettys, Palmetto Biotech).
      Western Blotting Analysis: A representative lot detected Stearoyl-CoA desaturase 1 in mouse microsomal liver preps (Hasek, B.E., et al. (2013). Diabetes. 62:1-12).
      Biological Information
      ImmunogenLinear peptide corresponding to mouse Stearoyl-CoA desaturase 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Scd1
      Purification MethodAffinity
      UniProt Number
      Molecular Weight~30 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.

      Western Blotting Analysis: A 1:100 dilution of this antibody detected Stearoyl-CoA desaturase 1 in 10 µg of microsomal preparation of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Stearoyl-CoA desaturase 1 -Q2556398 Q2556398

      References

      Reference overviewPub Med ID
      Remodeling the integration of lipid metabolism between liver and adipose tissue by dietary methionine restriction in rats.
      Hasek, BE; Boudreau, A; Shin, J; Feng, D; Hulver, M; Van, NT; Laque, A; Stewart, LK; Stone, KP; Wanders, D; Ghosh, S; Pessin, JE; Gettys, TW
      Diabetes 62 3362-72 2013

      Show Abstract
      23801581 23801581

      Brochure

      Title
      NPI Flyer- Epigenetics and Nuclear Function Feature (EMD)

      Technical Info

      Title
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