05-184 Anti-Src Antibody, clone GD11

05-184
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, H, M, Mi, R IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-184
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Src Antibody, clone GD11
      Background InformationSrc is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry.
      Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer.
      Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
      v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin or Non-stimulated human A431 cell lysate
      PresentationProtein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-Src Antibody, clone GD11 detects level of Src & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant chicken pp60src; v-src
      Epitopeamino acids 82-169
      CloneGD11
      HostMouse
      SpecificityRecognizes pp60src.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Human
      • Mouse
      • Mink
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • ASV
      • pp60c-src
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on RIPA lysates from non-stimulated A431 carcinoma cells, RIPA lysates from EGF-stimulated A431 cells, mouse 3T3/A31 cells or Cos-1 cells transfected with pUSE Mouse src
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Src, clone GD11 - 23989062398906
      Anti-Src, clone GD11 - 07020518020702051802
      Anti-Src, clone GD11 - 1645816458
      Anti-Src, clone GD11 - 1717317173
      Anti-Src, clone GD11 - 1726517265
      Anti-Src, clone GD11 - 1801618016
      Anti-Src, clone GD11 - 1866318663
      Anti-Src, clone GD11 - 1970519705
      Anti-Src, clone GD11 - 19826641982664
      Anti-Src, clone GD11 - 20376332037633

      References

      Reference overviewApplicationPub Med ID
      Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis.
      Tabariès, S; Annis, MG; Hsu, BE; Tam, CE; Savage, P; Park, M; Siegel, PM
      Oncotarget  6  9476-87  2015

      Show Abstract
      25823815 25823815
      STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.
      Sun, J; Lu, F; He, H; Shen, J; Messina, J; Mathew, R; Wang, D; Sarnaik, AA; Chang, WC; Kim, M; Cheng, H; Yang, S
      The Journal of cell biology  207  535-48  2014

      Show Abstract
      25404747 25404747
      Pre-clinical efficacy of PU-H71, a novel HSP90 inhibitor, alone and in combination with bortezomib in Ewing sarcoma.
      Ambati, SR; Lopes, EC; Kosugi, K; Mony, U; Zehir, A; Shah, SK; Taldone, T; Moreira, AL; Meyers, PA; Chiosis, G; Moore, MA
      Molecular oncology  8  323-36  2014

      Show Abstract
      24388362 24388362
      EGFR inhibitors exacerbate differentiation and cell cycle arrest induced by retinoic acid and vitamin D3 in acute myeloid leukemia cells.
      Lainey, E; Wolfromm, A; Sukkurwala, AQ; Micol, JB; Fenaux, P; Galluzzi, L; Kepp, O; Kroemer, G
      Cell cycle (Georgetown, Tex.)  12  2978-91  2013

      Show Abstract
      Western Blotting23974111 23974111
      Both Kdr and Flt1 play a vital role in hypoxia-induced Src-PLD1-PKCγ-cPLA(2) activation and retinal neovascularization.
      Singh, NK; Hansen, DE; Kundumani-Sridharan, V; Rao, GN
      Blood  121  1911-23  2013

      Show Abstract
      23319572 23319572
      The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics.
      Strizzi, L; Margaryan, NV; Gilgur, A; Hardy, KM; Normanno, N; Salomon, DS; Hendrix, MJ
      Cell cycle (Georgetown, Tex.)  12  1450-6  2013

      Show Abstract
      Western Blotting23574716 23574716
      12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration: a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction.
      Kundumani-Sridharan, V; Dyukova, E; Hansen, DE; Rao, GN
      The Journal of biological chemistry  288  15830-42  2013

      Show Abstract
      23589307 23589307
      Deltamethrin inhibits osteoclast differentiation via regulation of heme oxygenase-1 and NFATc1.
      Hiroshi Sakamoto,Eiko Sakai,Reiko Fumimoto,Yu Yamaguchi,Yutaka Fukuma,Kazuhisa Nishishita,Kuniaki Okamoto,Takayuki Tsukuba
      Toxicology in vitro : an international journal published in association with BIBRA  26  2012

      Show Abstract
      22634059 22634059
      Intracellular amyloid precursor protein sorting and amyloid-β secretion are regulated by Src-mediated phosphorylation of Mint2.
      Chaufty, J; Sullivan, SE; Ho, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  9613-25  2012

      Show Abstract
      22787047 22787047
      S-nitrosylation of c-Src via NMDAR-nNOS module promotes c-Src activation and NR2A phosphorylation in cerebral ischemia/reperfusion.
      Li-Juan Tang,Chong Li,Shu-Qun Hu,Yong-Ping Wu,Yan-Yan Zong,Chang-Cheng Sun,Fa Zhang,Guang-Yi Zhang
      Molecular and cellular biochemistry  365  2012

      Show Abstract
      22422045 22422045

      Technical Info

      Title
      Fluorescent Gelatin Degradation Assays for Investigating Invadopodia Formation

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2011, Vol. 2

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies