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05-184 | Anti-Src Antibody, clone GD11

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, H, M, Mi, R IP, WB M Purified Monoclonal Antibody
      Catalogue Number05-184
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Src Antibody, clone GD11
      Background InformationSrc is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry.
      Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer.
      Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
      v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.
      Product Information
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin or Non-stimulated human A431 cell lysate
      PresentationProtein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      ApplicationAnti-Src Antibody, clone GD11 detects level of Src & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant chicken pp60src; v-src
      Epitopeamino acids 82-169
      SpecificityRecognizes pp60src.
      Species Reactivity
      • Avian
      • Human
      • Mouse
      • Mink
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • ASV
      • pp60c-src
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on RIPA lysates from non-stimulated A431 carcinoma cells, RIPA lysates from EGF-stimulated A431 cells, mouse 3T3/A31 cells or Cos-1 cells transfected with pUSE Mouse src
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Src, clone GD11 - 2398906 2398906
      Anti-Src, clone GD11 - 0702051802 0702051802
      Anti-Src, clone GD11 - 16458 16458
      Anti-Src, clone GD11 - 17173 17173
      Anti-Src, clone GD11 - 17265 17265
      Anti-Src, clone GD11 - 18016 18016
      Anti-Src, clone GD11 - 18663 18663
      Anti-Src, clone GD11 - 19705 19705
      Anti-Src, clone GD11 - 1982664 1982664
      Anti-Src, clone GD11 - 2037633 2037633
      Anti-Src, clone GD11 - 20983 20983
      Anti-Src, clone GD11 - 21811 21811
      Anti-Src, clone GD11 - 2193143 2193143
      Anti-Src, clone GD11 - 22502 22502
      Anti-Src, clone GD11 - 2293827 2293827
      Anti-Src, clone GD11 - 23066 23066
      Anti-Src, clone GD11 - 24046 24046
      Anti-Src, clone GD11 - 25373 25373
      Anti-Src, clone GD11 - DAM1647860 DAM1647860
      Anti-Src, clone GD11 - DAM1658690 DAM1658690
      Anti-Src, clone GD11 - DAM1661024 DAM1661024
      Anti-Src, clone GD11 - DAM1745272 DAM1745272
      Anti-Src, clone GD11 - DAM1813162 DAM1813162
      Anti-Src, clone GD11 - JBC1870046 JBC1870046
      Anti-Src, clone GD11 - JBC1893942 JBC1893942
      Anti-Src, clone GD11 - JBC1900494 JBC1900494
      Anti-Src, clone GD11 -2491219 2491219
      Anti-Src, clone GD11 -2528706 2528706
      Anti-Src, clone GD11 -2578064 2578064
      Anti-Src, clone GD11 -2605367 2605367
      Anti-Src, clone GD11 -2637507 2637507
      Anti-Src, clone GD11 -2658754 2658754
      Anti-Src, clone GD11 -2688498 2688498
      Anti-Src, clone GD11 -2721712 2721712
      Anti-Src, clone GD11 -2780493 2780493
      Monoclonal Antibody - DAM1413928 DAM1413928

      References | 66 Available | See All References

      Reference overviewApplicationPub Med ID
      Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis.
      Tabariès, S; Annis, MG; Hsu, BE; Tam, CE; Savage, P; Park, M; Siegel, PM
      Oncotarget 6 9476-87 2015

      Show Abstract
      25823815 25823815
      STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion.
      Sun, J; Lu, F; He, H; Shen, J; Messina, J; Mathew, R; Wang, D; Sarnaik, AA; Chang, WC; Kim, M; Cheng, H; Yang, S
      The Journal of cell biology 207 535-48 2014

      Show Abstract
      25404747 25404747
      Pre-clinical efficacy of PU-H71, a novel HSP90 inhibitor, alone and in combination with bortezomib in Ewing sarcoma.
      Ambati, SR; Lopes, EC; Kosugi, K; Mony, U; Zehir, A; Shah, SK; Taldone, T; Moreira, AL; Meyers, PA; Chiosis, G; Moore, MA
      Molecular oncology 8 323-36 2014

      Show Abstract
      24388362 24388362
      EGFR inhibitors exacerbate differentiation and cell cycle arrest induced by retinoic acid and vitamin D3 in acute myeloid leukemia cells.
      Lainey, E; Wolfromm, A; Sukkurwala, AQ; Micol, JB; Fenaux, P; Galluzzi, L; Kepp, O; Kroemer, G
      Cell cycle (Georgetown, Tex.) 12 2978-91 2013

      Show Abstract
      Western Blotting23974111 23974111
      Both Kdr and Flt1 play a vital role in hypoxia-induced Src-PLD1-PKCγ-cPLA(2) activation and retinal neovascularization.
      Singh, NK; Hansen, DE; Kundumani-Sridharan, V; Rao, GN
      Blood 121 1911-23 2013

      Show Abstract
      23319572 23319572
      The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics.
      Strizzi, L; Margaryan, NV; Gilgur, A; Hardy, KM; Normanno, N; Salomon, DS; Hendrix, MJ
      Cell cycle (Georgetown, Tex.) 12 1450-6 2013

      Show Abstract
      Western Blotting23574716 23574716
      12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration: a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction.
      Kundumani-Sridharan, V; Dyukova, E; Hansen, DE; Rao, GN
      The Journal of biological chemistry 288 15830-42 2013

      Show Abstract
      23589307 23589307
      Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo.
      Lahlou, H; Sanguin-Gendreau, V; Frame, MC; Muller, WJ
      Breast cancer research : BCR 14 R36 2012

      Show Abstract
      Western Blotting22373082 22373082
      Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells.
      Hassan, HA; Cheng, M; Aronson, PS
      American journal of physiology. Cell physiology 302 C46-58 2012

      Show Abstract
      Immunoprecipitation21956166 21956166
      Deltamethrin inhibits osteoclast differentiation via regulation of heme oxygenase-1 and NFATc1.
      Hiroshi Sakamoto,Eiko Sakai,Reiko Fumimoto,Yu Yamaguchi,Yutaka Fukuma,Kazuhisa Nishishita,Kuniaki Okamoto,Takayuki Tsukuba
      Toxicology in vitro : an international journal published in association with BIBRA 26 2012

      Show Abstract
      22634059 22634059

      Technical Info

      Fluorescent Gelatin Degradation Assays for Investigating Invadopodia Formation

      Newsletters / Publications

      Cellutions Newsletter: 2011, Vol. 2

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      Alternative Format

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      Included Positive Control

      Catalog Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies