MAB1622 Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Gp, H, M, R, Rb, Po, Ma IHC, IP, WB, ChIP M Purified Monoclonal Antibody
      Catalogue NumberMAB1622
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6
      Alternate Names
      • Alpha-II spectrin
      • Fodrin alpha chain
      • Spectrin, non-erythroid alpha chain
      • non-erythrocytic spectrin alpha
      • spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
      • Fodrin α
      Background InformationNon-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.
      Product Information
      • HeLa cells treated with staurosporine
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.
      ApplicationAnti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (IFA):
      A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
      Does not work on paraffin sections.

      Optimal working dilutions must be determined by end user.

      Chromatin Immunopreciptiation:
      A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).
      Biological Information
      ImmunogenChicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificitySpecific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.
      Species Reactivity
      • Chicken
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Pig
      • Mammals
      Species Reactivity NoteChicken, Guinea Pig, Human, Mouse,Pig, Rat, and Rabbit. Wide-mammalian reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPTAN1
      • FLJ44613
      • SPTA2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13813 # Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
      SIZE:2472 amino acids; 284539 Da
      SUBUNIT: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with isoform 1 of ACP1.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
      PTM: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro (By similarity).
      SIMILARITY: SwissProt: Q13813 ## Belongs to the spectrin family. & Contains 3 EF-hand domains. & Contains 1 SH3 domain. & Contains 23 spectrin repeats.
      Molecular Weight~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on staurosporine-treated HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 12 months at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Fodrin, #225;, clone AA6 - LV1489729LV1489729
      Anti-Fodrin, , clone AA6 - JC1668385JC1668385
      Anti-Spectrin alpha chain2842101
      Anti-Spectrin alpha chain (nonerythroid), clone AA6 - 21159202115920
      Anti-Spectrin alpha chain (nonerythroid), clone AA6 - 21596092159609
      Anti-Spectrin alpha chain (nonerythroid), clone AA6 - 23874732387473
      Anti-Spectrin alpha chain (nonerythroid)2462798
      Anti-Spectrin alpha chain (nonerythroid), clone AA62901151
      Anti-Spectrin alpha chain (nonerythroid), clone AA6 - 19763881976388
      Anti-Spectrin alpha chain (nonerythroid), clone AA6 - 20045202004520


      Reference overviewApplicationSpeciesPub Med ID
      The sheddase ADAM10 is a potent modulator of prion disease.
      Altmeppen, HC; Prox, J; Krasemann, S; Puig, B; Kruszewski, K; Dohler, F; Bernreuther, C; Hoxha, A; Linsenmeier, L; Sikorska, B; Liberski, PP; Bartsch, U; Saftig, P; Glatzel, M
      eLife  4  2015

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      25654651 25654651
      Exclusion of the unfolded protein response in light-induced retinal degeneration in the canine T4R RHO model of autosomal dominant retinitis pigmentosa.
      Marsili, S; Genini, S; Sudharsan, R; Gingrich, J; Aguirre, GD; Beltran, WA
      PloS one  10  e0115723  2015

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      25695253 25695253
      EspC promotes epithelial cell detachment by enteropathogenic Escherichia coli via sequential cleavages of a cytoskeletal protein and then focal adhesion proteins.
      Navarro-Garcia, F; Serapio-Palacios, A; Vidal, JE; Salazar, MI; Tapia-Pastrana, G
      Infection and immunity  82  2255-65  2014

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      24643541 24643541
      Expression of Nampt in hippocampal and cortical excitatory neurons is critical for cognitive function.
      Stein, LR; Wozniak, DF; Dearborn, JT; Kubota, S; Apte, RS; Izumi, Y; Zorumski, CF; Imai, S
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  5800-15  2014

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      24760840 24760840
      Ammonia mediates methamphetamine-induced increases in glutamate and excitotoxicity.
      Halpin, LE; Northrop, NA; Yamamoto, BK
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  39  1031-8  2014

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      Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment.
      Hempel, C; Hoyer, N; Kildemoes, A; Jendresen, CB; Kurtzhals, JA
      Frontiers in immunology  5  291  2014

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      24995009 24995009
      Reduced migration of MLH1 deficient colon cancer cells depends on SPTAN1.
      Hinrichsen, I; Ernst, BP; Nuber, F; Passmann, S; Schäfer, D; Steinke, V; Friedrichs, N; Plotz, G; Zeuzem, S; Brieger, A
      Molecular cancer  13  11  2014

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      24456667 24456667
      Inhibition of calpain-regulated p35/cdk5 plays a central role in sildenafil-induced protection against chemical hypoxia produced by malonate.
      Barros-Miñones, L; Martín-de-Saavedra, D; Perez-Alvarez, S; Orejana, L; Suquía, V; Goñi-Allo, B; Hervias, I; López, MG; Jordan, J; Aguirre, N; Puerta, E
      Biochimica et biophysica acta  1832  705-17  2013

      Show Abstract
      23415811 23415811
      Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal aortic aneurysms in calpain-1 deficient mice.
      Subramanian, V; Moorleghen, JJ; Balakrishnan, A; Howatt, DA; Chishti, AH; Uchida, HA
      PloS one  8  e72214  2013

      Show Abstract
      23977256 23977256
      Prevention of JNK phosphorylation as a mechanism for rosiglitazone in neuroprotection after transient cerebral ischemia: activation of dual specificity phosphatase.
      Okami, N; Narasimhan, P; Yoshioka, H; Sakata, H; Kim, GS; Jung, JE; Maier, CM; Chan, PH
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  33  106-14  2013

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      Western Blotting23032483 23032483

      Data Sheet

      Anti-Fodrin, alpha, clone AA6 - Data Sheet

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