Key Specifications Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|Presentation||Liquid in PBS with 0.1% sodium azide.|
|Application||Detect Sp8 using this Anti-Sp8 Antibody validated for use in WB.|
|Application Notes||Western blot: 0.5-2 μg/mL on mouse brain lysate.
Optimal working dilutions must be determined by end user.
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.|
|Material Size||100 µg|
|Reference overview||Application||Species||Pub Med ID|
|Brain injury does not alter the intrinsic differentiation potential of adult neuroblasts. |
Liu, F; You, Y; Li, X; Ma, T; Nie, Y; Wei, B; Li, T; Lin, H; Yang, Z
The Journal of neuroscience : the official journal of the Society for Neuroscience 29 5075-87 2009
Neuroblasts produced by the neural stem cells of the adult subventricular zone (SVZ) migrate into damaged brain areas after stroke or other brain injuries, and previous data have suggested that they generate regionally appropriate new neurons. To classify the types of neurons produced subsequent to ischemic injury, we combined BrdU or virus labeling with multiple neuronal markers to characterize new cells at different times after the induction of stroke. We show that SVZ neuroblasts give rise almost exclusively to calretinin-expressing cells in the damaged striatum, resulting in the accumulation of these cells during long term recovery after stroke. The vast majority of SVZ neuroblasts as well as newly born young and mature neurons in the damaged striatum constitutively express the transcription factor Sp8, but do not express transcription factors characteristic of medium-sized spiny neurons, the primary striatal projection neurons lost after stroke. Our results suggest that adult neuroblasts do not alter their intrinsic differentiation potential after brain injury.
|Anti-Sp8 - Data Sheet|