PC347 Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb

PC347
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC347-25UG
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          Plastic ampoule 25 μg
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          Description
          OverviewRecognizes the serotonin 3 (5-HT3) receptor in mouse and rat brain tissue.
          Catalogue NumberPC347
          Brand Family Calbiochem®
          Application Data
          Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
          References
          ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
          Morales, M., et al. 1996. Brain Res. 731, 199.
          Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
          Maricq, A.V., et al. 1991. Science 254, 432.
          Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
          Derkach, V., et al. 1989. Nature 33, 706.
          Product Information
          FormLiquid
          Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
          Positive controlMouse or rat brain tissue
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Frozen Sections
          Not Western Blot
          Application NotesFrozen Sections (2-5 µg/ml)
          Immunoblotting (not recommended)
          Application CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC347

          References

          Reference overview
          Morales, M., et al. 1998. J. Comp. Neurol. 385, 385.
          Morales, M., et al. 1996. Brain Res. 731, 199.
          Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
          Maricq, A.V., et al. 1991. Science 254, 432.
          Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
          Derkach, V., et al. 1989. Nature 33, 706.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-October-2016 JSW
          ApplicationFrozen Sections (2-5 µg/ml)
          Immunoblotting (not recommended)
          Application Data
          Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the serotonin (5-HT3) receptor.
          BackgroundThe monoamine serotonin (5-hydroxytryptamine) mediates a variety of physiological actions by binding to a number of distinct receptors, pharmacologically classified into several major groups which differ in tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the 5-HT3 receptor is a ligand-gated ion channel that mediates a fast excitatory response. This receptor is a member of the superfamily of ligand-gated ion channels related to the nicotinic, GABAA and NMDA receptors. Although the 5-HT3 receptor was initially identified from the neuroblastoma cell line NCB-20, radioligand binding studies and in situ hybridization studies have localized the 5-HT3 receptor to a unique distribution of neurons, supporting possible roles for the receptor in cognition and sensory input. Recent immunohistochemical analysis of the rat brain revealed a broad distribution of 5-HT3 immunoreactivity, primarily including interneurons and motor neurons.
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
          IsotypeIgG
          Speciesmouse, rat
          Positive controlMouse or rat brain tissue
          FormLiquid
          Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
          Morales, M., et al. 1996. Brain Res. 731, 199.
          Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
          Maricq, A.V., et al. 1991. Science 254, 432.
          Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
          Derkach, V., et al. 1989. Nature 33, 706.