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PC176 | Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

PC176
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, R, Sh Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC176-100UL
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
          Catalogue NumberPC176
          Brand Family Calbiochem®
          Application Data
          Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
          References
          ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.
          Key Applications Free-floating Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesFrozen Sections (1:300-1:500)
          Immunoblotting (1:500-1:1000)
          Free Floating Sections (see application references)
          Paraffin Sections (see application references; heat pre-treatment required)
          Application CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
          ImmunogenRat
          HostRabbit
          Species Reactivity
          • Mouse
          • Rat
          • Sheep
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC176

          References

          Reference overview
          Leonard, B.E. 1996. Psychother. Psychosom. 65, 66.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-August-2007 JSW
          ApplicationFrozen Sections (1:300-1:500)
          Immunoblotting (1:500-1:1000)
          Free Floating Sections (see application references)
          Paraffin Sections (see application references; heat pre-treatment required)
          Application Data
          Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
          BackgroundAt least 14 mammalian serotonin receptor subtypes have been identified and classified into several families on the basis of common structural, pharmacological, and functional criteria. Serotonin (5-hydroxytryptamine [5-HT]) mediates its effects in a number of physiological processes including anxiety, depression, sexual activity, and sleep through interactions with different receptor subtypes. These receptors differ in their tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the serotonin 5-HT3 receptor acts as a cation-selective channel. The serotonin receptors have generated considerable pharmacological interest as targets for the identification of selective drugs that interact with a specific receptor subtype.
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
          Speciesmouse, rat, sheep
          FormLiquid
          FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
          Preservative≤0.1% sodium azide
          CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
          Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
          Application referencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.