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PC177L | Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

PC177L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      PC177L-100UL
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          Will advise
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
          Catalogue NumberPC177L
          Brand Family Calbiochem®
          Application Data
          Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
          References
          ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
          Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
          Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesFrozen Sections (1:10,000-1:15,000; see application references)
          Immunoblotting (see comments)
          Immunofluorescence (1:200-1:400)
          Application CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          PC177L

          References

          Reference overview
          Zhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
          Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
          Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 JSW
          ApplicationFrozen Sections (1:10,000-1:15,000; see application references)
          Immunoblotting (see comments)
          Immunofluorescence (1:200-1:400)
          Application Data
          Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
          DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
          BackgroundThe serotonin (5-hydroxytryptamine; 5-HT) transporter (5-HTT) plays an important role in the re-uptake of serotonin from the nerve terminal to ultimately terminate the serotonin signal. The 5-HTT plays a critical role in the regulation of serotonin neurotransmission, which has been linked to mood and personality disorders, anxiety, eating disorders, and insomnia. The predicted 12 transmembrane serotonin transporter contains potential sites for phosphorylation and glycosylation. Because serotonin is involved in such a variety of biological processes, the 5-HTT has been a target for the development of antidepressants and psychostimulants. Blocking serotonin uptake at the synapse has been achieved using structurally related chemicals called Selective Serotonin Re-uptake Inhibitors (SSRI) which alleviate disorders such as depression and alcoholism. The 5-HTT is localized to the plasma membrane of neurons and glial cells, as well as blood platelets, placenta, and endothelial cells.
          HostRabbit
          Immunogen speciesRat
          Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
          IsotypeIgG
          Speciesmouse, rat
          Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
          FormLyophilized
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
          Toxicity Standard Handling
          ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
          Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
          Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
          Application referencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.