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MABN1530 | Anti-Septin-3 Antibody, clone 5C1.2

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABN1530
      DescriptionAnti-Septin-3 Antibody, clone 5C1.2
      Alternate Names
      • Neuronal-specific septin-3E
      Background InformationNeuronal-specific septin-3 (UniProt Q9UH03) is encoded by the SEPT3 (also known as SEP3) gene (Gene ID 55964) in human. Septins are GTPases capable of forming higher-order oligomers and filamentous polymers that contact with cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. Septins exist in complexes that are composed of ordered non-polar arrays of multiple different septins. Human genome contains 13 septin genes that are grouped into the SEPT2 subgroup (septins 1, 2, 4, and 5), the SEPT3 subgroup (septins 3, 9, and 12), the SEPT6 subgroup (septins 6, 8, 10, 11, and 14), and the SEPT7 subgroup (septin 7). All subgroups contain the well conserved GTP-binding domain and differ mainly in their C-terminal region, where coiled-coil sequences are found in SEPT2, SEPT6, and SEPT7, but not SEPT3, subgroups. Septin-3 is the only septin to be expressed almost exclusively in brain tissue, where it is enriched in nerve terminals, suggesting a role in synaptic vesicle function. Increased septin-3 expression is reported in several brain tumours and SEPT3 polymorphisms are linked to Alzheimer's disease. PKG-1 is shown to phosphorylate septin-3 in vitro, non-phosphorylated septin-3 localizes to the peripheral membrane, whereas the phosphorylated form is found in the cytosol. Murine and rat septin-3 is reported to form complexes with septin-5 and septin-7.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
      ApplicationAnti-Septin-3, clone 5C1.2, Cat. No. MABN1153, is a highly specific mouse monoclonal antibody that targets Septin-3, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution of from a representative lot detected Septin-3 in 10 µg of human brain tissue lysate.

      Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Septin-3 in human cerebellum and cerebral cortex, as well as in mouse cerebral cortex tissue sections.
      Biological Information
      ImmunogenKLH-conjugatged linear peptide coresponding to an internal sequence from the N-terminal half of human septin-3.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 5C1.2 targets an internal epitope present in human spliced isoforms 1&2 (SEP3A and SEP3B), both mouse and all three rat spliced isoforms (G-septin-alpha, -beta, and -gamma) reporeted by UniProt (Q9UH03, Q9Z1S5, and Q9WU34, respectively). Target epitope is absent from human spliced isoform 3 (SEP3C).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SEPT3
      • SEP3
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~40/39 kDa observed. 40.70/40.10 kDa (human isoform 1/2), 40.04/38.71 kDa (mouse isoform 1/2), and 40.60/39.62/34.30 kDa (rat isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Septin-3 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information