DP01 Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419)

DP01
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Sv40 Infected Cells M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      DP01-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~94 kDa SV40 large T antigen and the ~21 kDa SV40 small T antigen.
          Catalogue NumberDP01
          Brand Family Calbiochem®
          References
          ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
          Whyte, P., et al. 1988 Nature 334, 124.
          Mitchell, P.J., et al.1987 Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J.1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Positive controlSV80 or SV-T2 cells
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.
          Key Applications Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesImmunoblotting (see application references)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenthe N-terminal domain of SV40 large and small T-antigen
          ClonePAb419
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Sv40 Infected Cells
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419) Certificates of Analysis

          TitleLot Number
          DP01

          References

          Reference overview
          DeCaprio, J.A., et al.1988. Cell 54, 275.
          Whyte, P., et al. 1988 Nature 334, 124.
          Mitchell, P.J., et al.1987 Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J.1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-August-2007 RFH
          ApplicationImmunoblotting (see application references)
          Immunofluorescence (1-5 µg/ml)
          Immunoprecipitation (1-2 µg/sample)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen and the ~20.5 kDa SV40 small T antigen.
          BackgroundSV40 T Ag (Ab-1) is a mouse monoclonal antibody with specificity for antigenic determinants localized to the amino terminal domain of the SV40 large T antigen. The sequences recognized by this reagent are also present on SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
          HostMouse
          Immunogenthe N-terminal domain of SV40 large and small T-antigen
          ClonePAb419
          IsotypeIgG2a
          SpeciesSV40 infected cells
          Positive controlSV80 or SV-T2 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
          Whyte, P., et al. 1988 Nature 334, 124.
          Mitchell, P.J., et al.1987 Cell 50, 847.
          Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
          Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
          Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
          Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
          Tooze, J.1973. Cold Spring Harbor, New York.
          Tegtmeyer, P. 1972. J. Virol. 10, 591.
          Application referencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.