A part of MilliporeSigma

MABC684 | Anti-SOD1 Antibody, clone 6F5

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC, FC M Ascites Monoclonal Antibody
      Catalogue NumberMABC684
      DescriptionAnti-SOD1 Antibody, clone 6F5
      Alternate Names
      • ALS
      • SOD
      • ALS1
      • IPOA
      • homodimerSuperoxide dismutase [Cu-Zn]
      • Superoxide dismutase 1
      • hSod1
      Background InformationSuperoxide dismutase 1 (SOD1) also known as Superoxide dismutase [Cu-Zn] to distinguish it from SOD2 which uses manganese as its cofactor, is the cytosolic form of the enzyme that catalyzes the breakdown of superoxide into oxygen and hydrogen peroxide and is our primary anti-oxidant combating enzyme. SOD1 performs its function with the help of co-factors and one protein in particular is vital to its proper functioning. Copper chaperone protein (CCS) is a metalloprotein that specifically delivers Cu to SOD1 and may activate SOD1 through direct insertion of the Cu cofactor. Interestingly, defects in SOD1 are the cause of a form of ALS (Amyotrophic Lateral Sclerosis or Lou Gehrig's disease) and much research is centered on understanding how over-active or under-active SOD1 contributes to the disease. EMD-Millipore's Anti-SOD1 mouse monoclonal antibody has been tested in western blot on HeLa, NIH3T3, A549 and A431 cell lysates and fluorescent immunocytochemistry on PANC1, SKBR-3 and 3T3-L1 cells in culture. The antibody has also been tested in flow cytometry on A431 cells in culture.
      Product Information
      • HeLa, NIH/3T3, A549, and A431 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      ApplicationAnti-SOD1 Antibody, clone 6F5 is a highly specific mouse monoclonal antibody, that targets Superoxide Dismutase & has been tested in western blotting, ICC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesImmunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SOD1 in PANC-1, SKBR-3, and 3T3-L1 cells.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SOD1 in non serum starved A431 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human SOD1 expressed in E. Coli.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SOD1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~18 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, NIH/3T3, A549, and A431 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SOD1 in HeLa, NIH/3T3, A549, and A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SOD1, clone 6F5 VP1406129
      Anti-SOD1, clone 6F5 - VP1402183 VP1402183
      Anti-SOD1, clone 6F5 - QVP1305200 QVP1305200
      Anti-SOD1, clone 6F5 - VP1310030 VP1310030
      Anti-SOD1, clone 6F5 -VP1509047 VP1509047
      Anti-SOD1, clone 6F5 -VP1509242 VP1509242
      Anti-SOD1, clone 6F5 -VP1511206 VP1511206
      Anti-SOD1, clone 6F5 -VP1601124 VP1601124
      Anti-SOD1, clone 6F5 -VP1607198 VP1607198
      Anti-SOD1, clone 6F5 -VP1609285 VP1609285


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies