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MABS164 | Anti-SHPS-1 Antibody, clone P84

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB, ICC, IHC, FUNC R Purified Monoclonal Antibody
      Catalogue NumberMABS164
      DescriptionAnti-SHPS-1 Antibody, clone P84
      Alternate Names
      • Tyrosine-protein phosphatase non-receptor type substrate 1
      • SHP substrate 1
      • Brain Ig-like molecule with tyrosine-based activation motifs
      • Bit
      • CD172 antigen-like family member A
      • Inhibitory receptor SHPS-1
      • MyD-1 antigen
      • Signal-regulatory protein alpha-1
      • Sirp-alpha-1
      • mSIRP-alpha1
      • p84
      • CD172a
      Background InformationSHPS-1 (also known as P84, BIT, and SIRP) is a neuronal membrane glycoprotein that promotes the attachment and neurite outgrowth of cultured murine cerebellar cells. Because of its heterophilic adhesive properties and its localization at synaptogenesis sites, SHPS-1 is suggested play a role in the regulation of synapse formation or maintenance. As a member of a phosphatase binding protein family, SHPS-1 shares identical homology with murine SHPS-1 cDNA. It is involved in receptor tyrosine kinase signaling that is found at synapses in the mammalian central nervous system and in non-neural tissues. It is reported that integrin-associated protein (IAP/CD47) is a predominant binding partner for P84. Upon phosphorylation of its cytoplasmic region, SHPS-1 binds to the protein-tyrosine phosphatase SHPS-2. Formation of the SHPS-1-SHP-2 complex is also implicated in the regulation of cellular migration. SHPS-1 and its ligand CD47 constitute an intercellular recognition system that contributes to the inhibition of cell migration via cell-cell contact. The ectodomain of SHPS-1 is shown to be excised from cells in a reaction that is likely mediated by a metalloproteinase. It is demonstrated that forced expression of a SHPS-1 mutant resistant to ectodomain shedding impaires cell migration, cell spreading, actin cytoskeleton reorganization, and paxillin- and FAK- tyrosine phosphorylation.
      Product Information
      • Mouse brain tissue lysate
      PresentationPurified rat monoclonal IgG1k in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-SHPS-1 Antibody, clone P84 detects level of SHPS-1 & has been published & validated for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse astrocytes (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232).
      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in adult mouse retina (Jiang, P., Lagenaur, C.F., and Narayanan, V. (1999). J Biol Chem. 274(2):559-562).
      Neurite Outgrowth Inhibition Analysis: A representative lot was used by an independent laboratory to inhibit neurite outgrowth in mouse cerebellum cells (Chuang, W., and Lagenaur, C.F., (1990). Developmental Biology. 137(2):219-232).
      Biological Information
      ImmunogenLinear peptide corresponding to mouse SHPS-1.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Sirpa
      • Bit
      • Myd1
      • Ptpns1
      • Shps1
      • Sirp
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

      SUBUNIT STRUCTURE: Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Highly expressed in cerebral cortex, brain, spinal cord, cerebellum and spleen, and at much lower levels in kidney, thymus, heart, lung and liver. Within the cerebellum, highly expressed throughout the molecular layer, and in synaptic glomeruli in the granule cell layer. Detected in neurons of the hippocampus and dentate gyrus, and in olfactory bulb. Not detected in Purkinje cells. Highly expressed in the plexiform layers, optic fiber layer and the outer segments of the photoreceptor layer in the retina. Highly expressed in macrophages. Isoform 3 is detected at very low levels in all tissues tested.
      Molecular Weight~100 kDa observed.
      The calculated molecular weight of this protein is 53 kDa, but can be observed at ~100-120 kDa due to high glycosolation (Comu, S., et al. (1997). J Neurosci. 17(22):8702-8710).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected SPHS-1 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information