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MABF860 | Anti-SAMHD1 Antibody, clone 4B5.1

MABF860
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMABF860
      DescriptionAnti-SAMHD1 Antibody, clone 4B5.1
      Alternate Names
      • dNTPase
      • SAMHD1
      • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
      • Dendritic cell-derived IFNG-induced protein
      • DCIP
      • Monocyte protein 5
      • MOP-5
      • SAM domain and HD domain-containing protein 1
      Background InformationDeoxynucleoside triphosphate triphosphohydrolase SAMHD1 (UniProt Q9Y3Z3; also known as DCIP, Dendritic cell-derived IFNG-induced protein, dNTPase, Monocyte protein 5, MOP-5, SAM domain and HD domain-containing protein 1) is encoded by the SAMHD1 (also known as CHBL2, HDDC1, MOP5, SBBI88) gene (Gene ID 25939) in human. The sterile alpha motif domain and histidine-aspartate domain-containing protein 1 (SAMHD1) is a dGTP/GTP-activated deoxynucleotide (dNTP) triphosphohydrolase regulated by Cyclin A2/CDK1-dependent phosphorylation and involved in balancing cellular dNTP pools. SAMHD1 was originally identified as a disease gene associated with the rare infantile encephalopathy Aicardi-Goutières syndrome, SAMHD1 was later shown to restrict HIV-1 replication in non-dividing myeloid-lineage cells and resting T cells by reducing the cellular dNTP concentration. Simian immunodeficiency viruses (SIVs) are found to use their Vpx/Vpr accessory proteins to engage and modify host Cullin-RING-type E3 ubiquitin ligases substrate recoginition to target SAMHD1 for poly-ubiquitylation and subsequent proteasomal degradation.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG1κ in supernatant without preservatives.
      Applications
      ApplicationThis Anti-SAMHD1 Antibody, clone 4B5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of SAMHD1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SAMHD1 in human small intestine tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminal of human SAMHD1.
      EpitopeN-terminal
      Clone4B5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all four spliced isoforms.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MOP5
      • SAMHD1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~72 kDa observed. 72.2 kDa (isoform 1), 69.4 kDa (isoform 2), 63.7 kDa (isoform 3), 68.1 kDa (isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected SAMHD1 in 10 µg of THP-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies