CB1039 Anti-S100A4 Mouse mAb (1F12-1G7)

CB1039
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      CB1039-100UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          100 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~10 kDa S100A4 protein in HeLa and NIH3T3 cells.
          Catalogue NumberCB1039
          Brand Family Calbiochem®
          SynonymsAnti-S100 Calcium Binding Protein A4
          Application Data
          Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Negative control293T cells
          Positive controlHeLa cells, NIH3T3 cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
          Clone1F12-1G7
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-S100A4 Mouse mAb (1F12-1G7) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-November-2010 RFH
          SynonymsAnti-S100 Calcium Binding Protein A4
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Application Data
          Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.

          Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified mouae monoclonal antibody. Recognizes the ~10 kDa S100A4 protein.
          BackgroundThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
          HostMouse
          ImmunogenFull-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein
          Clone1F12-1G7
          IsotypeIgG₁
          Specieshuman, mouse
          Positive controlHeLa cells, NIH3T3 cells
          Negative control293T cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review