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MABC653 | Anti-S100A4 Antibody, clone 9D5.1

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABC653
      DescriptionAnti-S100A4 Antibody, clone 9D5.1
      Alternate Names
      • Protein S100-A4
      • Calvasculin
      • Metastasin
      • Placental calcium-binding protein
      • Protein Mts1
      • S100 calcium-binding protein A4
      Background InformationProtein S100-A4 (UniProt P26447; also known as Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4) is encoded by the S100A4 (also known as CAPL, MTS1, P9KA, PEL98) gene (Gene ID 6275) in human. S100A4 belongs to a family of low moleclar weight proteins characterized by two calcium-binding helix-loop-helix EF-hand domains (a.a. 12-47 & 50-85 of human S100A4). S100 proteins exist as non-covalent homodimers with cell-specific expression patterns. S100A4 associates with cytoskeletal proteins, such as myosin and F-actin, as well as other actin-binding proteins such as Talin and integrins, and is involved in controlling not just shape and motility of normal cells, but also plays a role in cancer cells invasion/metastasis.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-S100A4 Antibody, clone 9D5.1 is an antibody against S100A4 for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A4 in HUVECs, HeLa, A431, and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant human S100A4 internal fragment.
      EpitopeWithin EF-hands 1&2.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone 9D5.1 was raised against human S100-A4 internal fragment that shares high sequence homology with the corresponding region in mouse (96%) and rat (93%) protein.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • S100A4
      • CAPL
      • MTS1
      • P9KA
      • PEL98
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~12 kDa oberved. 11.73/11.72/11.78 kDa (human/mouse/rat) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies