A part of MilliporeSigma

04-1054 | Anti-S100-β (CT) Antibody, clone EP1576Y, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IH(P), IP Rb Unpurified Monoclonal Antibody
      Catalogue Number04-1054
      DescriptionAnti-S100-β (CT) Antibody, clone EP1576Y, rabbit monoclonal
      Alternate Names
      • S-100 calcium-binding protein, beta chain
      • S-100 protein beta chain
      • S-100 protein subunit beta
      • S100 beta
      • S100 calcium binding protein B
      • S100 calcium binding protein, beta (neural)
      • S100 calcium-binding protein B
      • S100 calcium-binding protein, beta
      • S100 calcium-binding protein, beta (neural)
      Background InformationS-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
      Product Information
      • A375 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect S100-β (C-term) using this Anti-S100-β (C-term) Antibody, clone EP1576Y, Rabbit validated for use in WB, IH(P) & IP.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected S100-β in human melanoma tissue.
      Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues on the C-terminus of human S100-β.
      SpecificityThis antibody recognizes S100-β at and around the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
      Gene Symbol
      • NEF
      • OTTHUMP00000174958
      • S100
      • S100beta
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase.
      SUBUNIT STRUCTURE: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 By similarity. The S100B dimer interacts with two molecules of CAPZA1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      TISSUE SPECIFICITY: Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
      MISCELLANEOUS: In addition to metal-ion binding, this protein is involved with the regulation of protein phosphorylation in brain tissue.
      SEQUENCE SIMILARITIES: Belongs to the S-101 family.
      Contains 2 EF-hand domains.
      Molecular Weight~11 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A375 cell lysates.
      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect S100-β in A375 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - 1996261 1996261
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - 2102027 2102027
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - 2211049 2211049
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1664799 NG1664799
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1679587 NG1679587
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1703406 NG1703406
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1734252 NG1734252
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1858785 NG1858785
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NG1883342 NG1883342
      Anti-S100- (C-term), clone EP1576Y, Rabbit Monoclonal - NRG1629617 NRG1629617
      Anti-S100-#946; (C-term), clone EP1576Y, Rabbit Monoclonal - 2137049 2137049
      Anti-S100-β (C-term), clone EP1576Y, Rabbit Monoclonal - 2387721 2387721
      Anti-S100-β (C-term), clone EP1576Y, Rabbit Monoclonal - 2458922 2458922
      Anti-S100-β (C-term), clone EP1576Y, Rabbit Monoclonal - 2464916 2464916
      Anti-S100-β (C-term), clone EP1576Y, Rabbit Monoclonal -2488742 2488742


      Reference overviewApplicationPub Med ID
      Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?
      Kennedy, E; Hakimjavadi, R; Greene, C; Mooney, CJ; Fitzpatrick, E; Collins, LE; Loscher, CE; Guha, S; Morrow, D; Redmond, EM; Cahill, PA
      Vascular cell 6 6 2014

      Show Abstract
      24628920 24628920
      Chronic-pain-associated astrocytic reaction in the spinal cord dorsal horn of human immunodeficiency virus-infected patients.
      Shi, Y; Gelman, BB; Lisinicchia, JG; Tang, SJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 10833-40 2012

      Show Abstract
      Western Blotting22875918 22875918

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