A part of MilliporeSigma

MAB079-1 | Anti-S-100 Protein Antibody, clone 15E2E2

100 µL  @ 1 mg/mL concentation
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, M, R, Ca, Rb, Fe IHC, IH(P), IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB079-1
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-S-100 Protein Antibody, clone 15E2E2
      Alternate Names
      • Calcium Binding Protein A1/B1
      Background InformationS-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
      Product Information
      • Positive Control Tissue: Melanoma
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing PBS, pH 7.6, 1% BSA and 0.09% sodium azide.
      ApplicationAnti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      A previous lot of this antibody was used in IP.

      Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.

      A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified bovine S-100 protein
      EpitopeRecognizes both alpha & beta S100
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes intact, alpha and beta subunits of bovine, human, and murine S-100 protein, as determined by immunoprecipitation profiles.
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      • Rat
      • Canine
      • Rabbit
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
      Gene Symbol
      • S100A1
      • S100A
      • S100
      • S100-alpha
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
      SIZE: 94 amino acids; 10546 Da
      SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
      TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
      SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on PC12 lysates

      Western Blot Analysis:
      1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Material Package@ 1 mg/mL concentation
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-S-100 Protein, clone 15E2E2 - 2118933 2118933
      Anti-S-100 Protein, clone 15E2E2 - 2395675 2395675
      Anti-S-100 Protein, clone 15E2E2 - 2424627 2424627
      Anti-S-100 Protein, clone 15E2E2 - 2459010 2459010
      Anti-S-100 Protein, clone 15E2E2 - NG1820157 NG1820157
      Anti-S-100 Protein, clone 15E2E2 - 2021712 2021712
      Anti-S-100 Protein, clone 15E2E2 - 2053334 2053334
      Anti-S-100 Protein, clone 15E2E2 - 2172692 2172692
      Anti-S-100 Protein, clone 15E2E2 - 2273794 2273794
      Anti-S-100 Protein, clone 15E2E2 - 2302150 2302150
      Anti-S-100 Protein, clone 15E2E2 - LV1557488 LV1557488
      Anti-S-100 Protein, clone 15E2E2 - NG1869825 NG1869825
      Anti-S-100 Protein, clone 15E2E2 - NG1944968 NG1944968
      Anti-S-100 Protein, clone 15E2E2 - NMM1609424 NMM1609424
      Anti-S-100 Protein, clone 15E2E2 - NMM1662405 NMM1662405
      Anti-S-100 Protein, clone 15E2E2 - NMM1701223 NMM1701223
      Anti-S-100 Protein, clone 15E2E2 - NMM1724156 NMM1724156
      Anti-S-100 Protein, clone 15E2E2 -2580698 2580698
      Anti-S-100 Protein, clone 15E2E2 -2677886 2677886
      Anti-S-100 Protein, clone 15E2E2 -2701607 2701607
      Anti-S-100 Protein, clone 15E2E2 -2718301 2718301
      Anti-S-100 Protein, clone 15E2E2 -2739426 2739426
      Anti-S-100 Protein, clone 15E2E2 -2772789 2772789


      Reference overviewPub Med ID
      SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.
      Müller, C; Hochhaus, NM; Fontana, X; Luhmann, HJ; White, R
      PloS one 10 e0136900 2015

      Show Abstract
      26317513 26317513
      Spinal nerve ligation decreases γ-aminobutyric acidB receptors on specific populations of immunohistochemically identified neurons in L5 dorsal root ganglion of the rat.
      Engle, MP; Merrill, MA; Marquez De Prado, B; Hammond, DL
      The Journal of comparative neurology 520 1663-77 2012

      Show Abstract
      22120979 22120979
      RAGE-TXNIP axis is required for S100B-promoted Schwann cell migration, fibronectin expression and cytokine secretion.
      Sbai, O; Devi, TS; Melone, MA; Feron, F; Khrestchatisky, M; Singh, LP; Perrone, L
      Journal of cell science 123 4332-9 2010

      Show Abstract
      21098642 21098642
      Giant schwannoma of the cheek--a comprehensive histological and immunohistochemical description of a rare tumour.
      Mariusz Gajda, Olaf Zagólski, Agnieszka Jasztal, Grzegorz J Lis, Dariusz Adamek, Jan A Litwin, Mariusz Gajda, Olaf Zagólski, Agnieszka Jasztal, Grzegorz J Lis, Dariusz Adamek, Jan A Litwin
      Polish journal of pathology : official journal of the Polish Society of Pathologists 60 52-6 2009

      Show Abstract
      19670705 19670705
      Immunohistochemical visualization of neurons and specific glial cells for stereological application in the porcine neocortex.
      Lise Lyck, Jacob Jelsing, Pia Søndergaard Jensen, Kate Lykke Lambertsen, Bente Pakkenberg, Bente Finsen
      Journal of neuroscience methods 152 229-42 2006

      Show Abstract
      16269187 16269187
      Satellite cells of dorsal root ganglia are multipotential glial precursors.
      Svenningsen, Asa Fex, et al.
      Neuron Glia Biol., 1: 85-93 (2004) 2004

      18634609 18634609
      Schwann cell apoptosis in the postnatal axotomized sciatic nerve is mediated via NGF through the low-affinity neurotrophin receptor.
      Petratos, Steven, et al.
      J. Neuropathol. Exp. Neurol., 62: 398-411 (2003) 2003

      Show Abstract
      12722832 12722832
      Neurotrophins and other growth factors in the regenerative milieu of proximal nerve stump tips.
      Zochodne, D W and Cheng, C
      J. Anat., 196 ( Pt 2): 279-83 (2000) 2000

      Show Abstract
      10739024 10739024
      Cellular immunolocalization of S100 protein within fixed tissue sections by monoclonal antibodies.
      Loeffel, S C, et al.
      Arch. Pathol. Lab. Med., 109: 117-22 (1985) 1985

      2579618 2579618

      Data Sheet

      Anti-S-100 Protein, recognizes both alpha & beta S100, clone 15E2-E2-A1 - Data Sheet