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NR07 | Anti-Ryanodine Receptor Mouse mAb (C3-33)

NR07
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Am, Av, Ca, F, Rb, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      NR07-100UG
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      Discontinued
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.
          Catalogue NumberNR07
          Brand Family Calbiochem®
          Application Data

          Detection of rat ryanodine receptor by immunocytochemistry. Sample: Rat brain hippocampus. Primary antibody: Anti-Ryanodine Receptor Mouse mAb (C3-33) (Cat. No. NR07) (1:50). Detection: fluorescence.
          References
          ReferencesSitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
          McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
          Meszaros, L.G., et al. 1993. Nature 364, 76.
          Lai, F.A., et al. 1992. Biochem. J. 288, 553.
          Lai, F.A., et al. 1989. Nature 331, 315.
          Product Information
          FormLiquid
          FormulationAscites diluted in PBS.
          Positive controlRat brain hippocampus
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Lai, F.A., et al. 1992. Amer. J. Physiol. 263, C365. Frozen Sections Junker, J., et al. 1994. J. Biol. Chem. 269, 1627.
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesFrozen Sections (1 µg/ml, see application references)
          Immunoblotting (1 µg/ml)
          Immunoprecipitation (see comments)
          Application CommentsA ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogencanine cardiac ryanodine receptor (RYR)
          ImmunogenCanine
          CloneC3-33
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Amphibian
          • Avian
          • Canine
          • Fish
          • Rabbit
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          NR07

          References

          Reference overview
          Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
          McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
          Meszaros, L.G., et al. 1993. Nature 364, 76.
          Lai, F.A., et al. 1992. Biochem. J. 288, 553.
          Lai, F.A., et al. 1989. Nature 331, 315.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-September-2007 JSW
          ApplicationFrozen Sections (1 µg/ml, see application references)
          Immunoblotting (1 µg/ml)
          Immunoprecipitation (see comments)
          Application Data

          Detection of rat ryanodine receptor by immunocytochemistry. Sample: Rat brain hippocampus. Primary antibody: Anti-Ryanodine Receptor Mouse mAb (C3-33) (Cat. No. NR07) (1:50). Detection: fluorescence.
          DescriptionMouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as diluted ascites. Recognizes the ~565 kDa ryanodine receptor.
          BackgroundPrecise regulation of the intracellular calcium level plays a critical role in numerous brain functions. The identification of intracellular calcium-release channels was aided by the biochemical use of ryanodine, a neutral plant alkaloid known to effect calcium release from the sarcoplasmic reticulum. Ryanodine receptors were originally identified in the sarcoplasmic reticulum of skeletal and cardiac muscle. Three distinct receptor subtypes have since been characterized and include RyR1, RyR2, and RyR3. RyR1 is predominantly expressed in skeletal muscle, but is also localized to Purkinje cells in the cerebellum. RyR2 is primarily expressed in the heart, although expression has been localized to stomach, endothelial cells, and the brain. RyR2 shows widespread neuronal distribution, and is the primary form of the ryanodine receptor expressed in the brain. RyR3 is expressed in the striatum, thalamus, and hippocampus of the brain, as well as smooth muscle and epithelial cells. In non-skeletal muscle, several agonists have been proposed to open the RyR, including calcium or cADP-ribose. The distribution of the ryanodine receptor within the mammalian central nervous system is overlapping but distinct from the inositol 1,4,5-triphosphate receptors (IP3Rs) which are also involved in intracellular Ca2+ release.
          HostMouse
          Immunogen speciesCanine
          Immunogencanine cardiac ryanodine receptor (RYR)
          CloneC3-33
          IsotypeIgG₁
          Speciesamphibian, avian, canine, fish, rabbit (weakly), rat
          Positive controlRat brain hippocampus
          FormLiquid
          FormulationAscites diluted in PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsA ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesSitsapesan, R., et al. 1995. Trends Pharmacol. Sci. 16, 386.
          McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem. 268, 13765.
          Meszaros, L.G., et al. 1993. Nature 364, 76.
          Lai, F.A., et al. 1992. Biochem. J. 288, 553.
          Lai, F.A., et al. 1989. Nature 331, 315.
          Application referencesOriginal Clone Lai, F.A., et al. 1992. Amer. J. Physiol. 263, C365. Frozen Sections Junker, J., et al. 1994. J. Biol. Chem. 269, 1627.