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MABN804 | Anti-Ribeye Antibody, clone 3D12.1

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABN804
      DescriptionAnti-Ribeye Antibody, clone 3D12.1
      Alternate Names
      • C-terminal-binding protein 2
      • CtBP2
      • Ribeye
      Background InformationHuman C-terminal-binding protein 2 (CtBP2) and Ribeye (UniProt P56545-1 and P56545-2, respectively) are two proteins encoded by the CTBP2 gene (Gene ID 1488) in human, they are produced as a result of alternative splicing. The C-terminal 425-amino acid sequence of Ribeye (aa 561-985) is identical to that of CtBP2 (aa 21-445), however the first 20 amino acids at the CtBP2 N-terminal end is replaced with a 560-amino acid sequence in the case of Ribeye. Ribeye is a protein specific to synaptic ribbons. Ribbon synapses are high-performance synapses built by certain nonspiking sensory neurons, such as photoreceptors, retinal bipolar cells, and inner ear hair cells. The main site of exocytosis of these neurons is characterized by unique presynaptic structures called the synaptic ribbons that associate with large numbers of synaptic vesicles primed for exocytosis and has a direct impact on synaptic signaling. Ribeye is composed of an N-terminal A domain and a C-terminal B domain that is largely identical to CtBP2. The A domain unique to Ribeye has a structural role at the synaptic ribbon, while the B domain of Ribeye possesses lysophosphatidic acid (LPA) acyltransferase (LPAAT) activity that catalyzes the generation of the highly active signaling phospholipid phosphatidic acid (PA) from LPA. LPAAT-deficient Ribeye mutant does not recruit PA-binding proteins to synaptic ribbons, whereas wild-type RIBEYE supports PA-dependent binding, The B domain also contains an NADH-binding subdomain (NBD), which is reported to compete against Arf1 for Arf-GTPase activating protein-3 (ArfGAP3) binding. Ribeye therefore functions as an Arf1 positive regulator at the synaptic ribbon by preventing the inactivation of Arf1 by ArfGAP3.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Ribeye Antibody, clone 3D12.1, Cat. No. MABN804, is a highly specific mouse monoclonal antibody, that targets Ribeye and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Ribeye in 10 µg of SH-SY5Y cell lysate.
      Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Ribeye in human cardiac myocytes and human cerebellum tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminal region of human Ribeye.
      EpitopeN-terminal region
      ConcentrationPlease refer to lot specific datasheet.
      SpecificitySpecific for CTBP2 spliced variant Ribeye. Does not react with the canonical CtBP2 isoform.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTBP2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~106 kDa observed (109.2 kDa calculated).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ribeye in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information