A part of MilliporeSigma

05-723 | Anti-RhoE/Rnd3 Antibody, clone 4

05-723
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, WB M Ascites Monoclonal Antibody
      Description
      Catalogue Number05-723
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RhoE/Rnd3 Antibody, clone 4
      Alternate Names
      • MemB protein
      • Rho family GTPase 3
      • ras homolog gene family, member E
      • small GTP binding protein Rho8
      Background InformationRhoE, also known as Rnd3, (Rho family GTPase 3) is a small G-protein that is a member of the Rnd family of Rho GTPases, which is a part of the Ras superfamily. The activity of Rnd1, Rnd2, and Rnd3/RhoE are not activated by the traditional conformational change of the GTP binding like other Rho family members. As the name dictates, Rnd proteins were associated with making a cell “round”. Like other small G-proteins, the do bind GTP, but unlike other G-proteins, they do not hydrolyze GTP to GDP. The actions of RhoE are associated with the actin cytoskeletal organization in response to external stimuli. RhoE acts antagonistically to RhoA in inducing loss of stress fiber formation, in part by binding to the RhoA downstream kinase Rho-associated kinase (ROCK1). This prevents ROCK1 activation and inhibits downstream signaling. RhoE does go through the post-translation modification of farnylation which enables it to localize and bind to various internal membranes. RhoE is also known to effect cycle progression.
      References
      Product Information
      FormatAscites
      Control
      • Included Positive Antigen Control: Catalog #12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationMouse monoclonal ascites IgG in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
      Applications
      ApplicationAnti-RhoE/Rnd3 Antibody, clone 4 is an antibody against RhoE/Rnd3 for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      A 1:50 dilution of this antibody has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100 (Riento, K., 2003).
      Biological Information
      ImmunogenFull length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3. Clone 4.
      Clone4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes RhoE/Rnd3, Mr 29 kDa. Weakly cross-reacts with Rnd1, Mr 27 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM
      Gene Symbol
      • RND3
      • RHOE
      • RhoE
      • Rho8
      • memB
      • ARHE
      • RHO8
      • Rnd3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P61587 # Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
      SIZE: 244 amino acids; 27368 Da
      SUBUNIT: Binds ROCK1 (By similarity). Interacts with UBXD5.
      SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein.
      TISSUE SPECIFICITY: Ubiquitous.
      SIMILARITY: SwissProt: P61587 ## Belongs to the small GTPase superfamily. Rho family.
      Molecular WeightRhoE/Rnd3 (~29 kDa); Rnd1 (~27 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.

      Western Blot Analysis:
      A 1:500-1:2000 dilution of this lot detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RhoE/Rnd3, clone 4 - 2430530 2430530
      Anti-RhoE/Rnd3, clone 4 - 1969118 1969118
      Anti-RhoE/Rnd3, clone 4 - 2044815 2044815
      Anti-RhoE/Rnd3, clone 4 - 2250528 2250528
      Anti-RhoE/Rnd3, clone 4 - 24565 24565
      Anti-RhoE/Rnd3, clone 4 - 32196 32196
      Anti-RhoE/Rnd3, clone 4 - DAM1411330 DAM1411330
      Anti-RhoE/Rnd3, clone 4 - DAM1501798 DAM1501798
      Anti-RhoE/Rnd3, clone 4 -2586449 2586449
      Anti-RhoE/Rnd3, clone 4 -2697429 2697429
      Anti-RhoE/Rnd3, clone 4 -2717629 2717629
      Anti-RhoE/Rnd3, clone 4 -2739283 2739283
      Anti-RhoE/Rnd3, clone 4 -2802018 2802018

      References

      Reference overviewPub Med ID
      Rnd3/RhoE Is down-regulated in hepatocellular carcinoma and controls cellular invasion.
      Grise, Florence, et al.
      Hepatology, 55: 1766-75 (2012) 2012

      Show Abstract
      22234932 22234932
      A switch in RND3-RHOA signaling is critical for melanoma cell invasion following mutant-BRAF inhibition.
      Klein, RM; Higgins, PJ
      Molecular cancer 10 114 2011

      Show Abstract Full Text Article
      21917148 21917148
      B-RAF regulation of Rnd3 participates in actin cytoskeletal and focal adhesion organization.
      Klein, RM; Spofford, LS; Abel, EV; Ortiz, A; Aplin, AE
      Molecular biology of the cell 19 498-508 2008

      Show Abstract Full Text Article
      18045987 18045987
      Expression of RND proteins in human myometrium.
      Lartey, J; Gampel, A; Pawade, J; Mellor, H; Bernal, AL
      Biology of reproduction 75 452-61 2006

      Show Abstract
      16554414 16554414
      RhoE binds to ROCK I and inhibits downstream signaling.
      Riento, Kirsi, et al.
      Mol. Cell. Biol., 23: 4219-29 (2003) 2003

      Show Abstract
      12773565 12773565

      Related Products & Applications

      Included Positive Control

      Catalog Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies