A part of MilliporeSigma

AB3768 | Anti-Rad54L Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ICC, IHC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberAB3768
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Rad54L Antibody
      Product Information
      FormatAffinity Purified
      PresentationPrecipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

      PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
      ApplicationAnti-Rad54L Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Rad54L & has been validated in ICC & IHC.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:200-1:1,000.

      Immunocytochemistry: 1:200-1:1,000.

      Optimal working dilutions must be determined by the end user.SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY

      1. Dissect tissues and freeze on dry ice

      2. Cut on a cryostat - 10um slices

      3. Dry slides at room temperature for 30-90 min

      4. Fix with cold Acetone/Methanol (50/50) 2 min - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin , incubation 10 minutes at 37°C, followed by 3 washes with PBS)

      5. Let air dry

      6. PBS 5min

      7. Incubate in 50mM ammonium chloride 30 min

      8. PBS 5min

      9. Blocking serum 30-45min

      10. Primary antibody 90min - dilution 1:100 - 1:600

      11. Wash 3 times for 5 min

      12. Secondary fluorescent conjug. antibody 30min

      13. Wash 3 times for 5 min and mount
      Biological Information
      ImmunogenSynthetic peptide, amino acids 12-27 of RAD51L.
      SpecificityRecognizes RAD54L. The molecular weight of the protein is 84,326 Daltons.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination.
      Gene Symbol
      • RAD54L
      • HR54
      • hRAD54
      • RAD54A
      • hHR54
      • HRAD54
      • EC 3.6.1.-
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92698 # Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
      SIZE: 747 amino acids; 84352 Da
      SUBUNIT: Interacts with RAD51 through the NH2-terminal domain.
      DISEASE:SwissProt: Q92698 # Defects in RAD54L may be a cause of tumor formation. Although alterations are found in a small fraction of primary tumors, these findings provide new insight into genetic instability underlying carcinogenesis.
      SIMILARITY: SwissProt: Q92698 ## Belongs to the SNF2/RAD54 helicase family. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


      Reference overviewApplicationPub Med ID
      Two nested developmental waves demarcate a compartment boundary in the mouse lung.
      Alanis, DM; Chang, DR; Akiyama, H; Krasnow, MA; Chen, J
      Nature communications 5 3923 2014

      Show Abstract
      24879355 24879355
      Lung epithelial branching program antagonizes alveolar differentiation.
      Chang, DR; Martinez Alanis, D; Miller, RK; Ji, H; Akiyama, H; McCrea, PD; Chen, J
      Proceedings of the National Academy of Sciences of the United States of America 110 18042-51 2013

      Show Abstract
      Immunohistochemistry24058167 24058167

      Data Sheet

      Anti-Rad54L - Data Sheet