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05-389 | Anti-Rac1 Antibody, clone 23A8

250 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, IP, WB M Purified Monoclonal Antibody
      Catalogue Number05-389
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rac1 Antibody, clone 23A8
      Background InformationRac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
      Product Information
      • Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
        Immunohistochemistry: Rat brain sections
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationThis Anti-Rac1 Antibody, clone 23A8 has been published in more than 40 citations and validated for use in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Rac1 protein.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein containing the full length human Rac
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
      Gene Symbol
      • RAC1
      • MGC111543
      • ORFNames=MIG5
      • MIG5
      • TC-25
      • p21-Rac1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
      SIZE: 192 amino acids; 21450 Da
      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
      DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight21 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on rat brain microsomal protein preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rac1, clone 23A8 2476975
      Anti-Rac1, clone 23A8 - 2116619 2116619
      Anti-Rac1, clone 23A8 - 2382127 2382127
      Anti-Rac1, clone 23A8 - 2397127 2397127
      Anti-Rac1, clone 23A8 - 2452510 2452510
      Anti-Rac1, clone 23A8 - 15254 15254
      Anti-Rac1, clone 23A8 - 16999 16999
      Anti-Rac1, clone 23A8 - 18070 18070
      Anti-Rac1, clone 23A8 - 18423 18423
      Anti-Rac1, clone 23A8 - 18826 18826
      Anti-Rac1, clone 23A8 - 1969117 1969117
      Anti-Rac1, clone 23A8 - 1973420 1973420
      Anti-Rac1, clone 23A8 - 2018111 2018111
      Anti-Rac1, clone 23A8 - 20815 20815
      Anti-Rac1, clone 23A8 - 21954 21954
      Anti-Rac1, clone 23A8 - 2197496 2197496
      Anti-Rac1, clone 23A8 - 2328346 2328346
      Anti-Rac1, clone 23A8 - 23588 23588
      Anti-Rac1, clone 23A8 - 2494036 2494036
      Anti-Rac1, clone 23A8 - 26345 26345
      Anti-Rac1, clone 23A8 - 27666 27666
      Anti-Rac1, clone 23A8 - DAM1469465 DAM1469465
      Anti-Rac1, clone 23A8 - DAM1522857 DAM1522857
      Anti-Rac1, clone 23A8 - DAM1604948 DAM1604948
      Anti-Rac1, clone 23A8 - DAM1655243 DAM1655243
      Anti-Rac1, clone 23A8 - DAM1690618 DAM1690618
      Anti-Rac1, clone 23A8 - DAM1713162 DAM1713162
      Anti-Rac1, clone 23A8 - DAM1739151 DAM1739151
      Anti-Rac1, clone 23A8 - DAM1749917 DAM1749917
      Anti-Rac1, clone 23A8 - DAM1787840 DAM1787840
      Anti-Rac1, clone 23A8 - DAM1821046 DAM1821046
      Anti-Rac1, clone 23A8 - JBC1350127 JBC1350127
      Anti-Rac1, clone 23A8 - JBC1863405 JBC1863405
      Anti-Rac1, clone 23A8 - JBC1881336 JBC1881336
      Anti-Rac1, clone 23A8 -2510309 2510309
      Anti-Rac1, clone 23A8 -2551207 2551207
      Anti-Rac1, clone 23A8 -2585802 2585802
      Anti-Rac1, clone 23A8 -2586803 2586803
      Anti-Rac1, clone 23A8 -2639388 2639388
      Anti-Rac1, clone 23A8 -2697414 2697414
      Anti-Rac1, clone 23A8 -2727207 2727207
      Anti-Rac1, clone 23A8 -2768482 2768482

      References | 105 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Lichen Secondary Metabolite, Physciosporin, Inhibits Lung Cancer Cell Motility.
      Yang, Y; Park, SY; Nguyen, TT; Yu, YH; Nguyen, TV; Sun, EG; Udeni, J; Jeong, MH; Pereira, I; Moon, C; Ha, HH; Kim, KK; Hur, JS; Kim, H
      PloS one 10 e0137889 2015

      Show Abstract
      26371759 26371759
      Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.
      Poitelon, Y; Bogni, S; Matafora, V; Della-Flora Nunes, G; Hurley, E; Ghidinelli, M; Katzenellenbogen, BS; Taveggia, C; Silvestri, N; Bachi, A; Sannino, A; Wrabetz, L; Feltri, ML
      Nature communications 6 8303 2015

      Show Abstract
      Immunohistochemistry26383514 26383514
      The proto-oncogene c-Src and its downstream signaling pathways are inhibited by the metastasis suppressor, NDRG1.
      Liu, W; Yue, F; Zheng, M; Merlot, A; Bae, DH; Huang, M; Lane, D; Jansson, P; Lui, GY; Richardson, V; Sahni, S; Kalinowski, D; Kovacevic, Z; Richardson, DR
      Oncotarget 6 8851-74 2015

      Show Abstract
      25860930 25860930
      Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms.
      Hinterseher, I; Schworer, CM; Lillvis, JH; Stahl, E; Erdman, R; Gatalica, Z; Tromp, G; Kuivaniemi, H
      International journal of molecular sciences 16 11196-212 2015

      Show Abstract
      25993291 25993291
      ROCK1 deficiency enhances protective effects of antioxidants against apoptosis and cell detachment.
      Surma, M; Handy, C; Chang, J; Kapur, R; Wei, L; Shi, J
      PloS one 9 e90758 2014

      Show Abstract
      Western Blotting24595357 24595357
      Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
      Moon, S; Han, D; Kim, Y; Jin, J; Ho, WK; Kim, Y
      Scientific reports 4 4376 2014

      Show Abstract
      Western Blotting24625528 24625528
      Downregulation of microRNA-100 enhances the ICMT-Rac1 signaling and promotes metastasis of hepatocellular carcinoma cells.
      Zhou, HC; Fang, JH; Luo, X; Zhang, L; Yang, J; Zhang, C; Zhuang, SM
      Oncotarget 5 12177-88 2014

      Show Abstract
      Western BlottingMouse25361001 25361001
      Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength.
      Bentzinger, CF; von Maltzahn, J; Dumont, NA; Stark, DA; Wang, YX; Nhan, K; Frenette, J; Cornelison, DD; Rudnicki, MA
      The Journal of cell biology 205 97-111 2014

      Show Abstract
      Immunofluorescence24711502 24711502
      Novel mechanism of JNK pathway activation by adenoviral E1A.
      Romanov, VS; Brichkina, AI; Morrison, H; Pospelova, TV; Pospelov, VA; Herrlich, P
      Oncotarget 5 2176-86 2014

      Show Abstract
      24742962 24742962
      EZH2 regulates cofilin activity and colon cancer cell migration by targeting ITGA2 gene.
      Ferraro, A; Boni, T; Pintzas, A
      PloS one 9 e115276 2014

      Show Abstract
      Western Blotting25549357 25549357

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