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04-1570 | Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

04-1570
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ChIP, WB, ELISA R Purified Monoclonal Antibody
      Description
      Catalogue Number04-1570
      DescriptionAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
      Alternate Names
      • DNA-directed RNA polymerase II A
      • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA polymerase II subunit B1
      • RNA-directed RNA polymerase II subunit RPB1
      • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
      • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
      Background InformationRNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.
      References
      Product Information
      FormatPurified
      Control
      • γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 (phospho-CTD Ser-7) has been validated in WB, ELISA.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • ELISA
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser7.
      EpitopeSer7
      Clone4E12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityThis antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser7.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq].
      Gene Symbol
      • POLR2A
      • POLR2
      • POLRA
      • RPB1
      • RPBh1
      • RPO2
      • RPOL2
      • RpIILS
      • hRPB220
      • hsRPB1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

      CATA;YTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

      SUBUNIT STRUCTURE: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2.

      SUBCELLULAR LOCATION: Nucleus

      PTM: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a "CTD code" that specifies the position of Pol II within the transcription cycle has been proposed.

      MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucelotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

      SEQUENCE SIMILARITIES: Belongs to the RNA polymerase beta' chain family.
      Molecular Weight~ 220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.

      Western Blot Analysis: 0.25 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2360004 2360004
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2433425 2433425
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2459058 2459058
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 1951949 1951949
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2052354 2052354
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2153072 2153072
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - 2495661 2495661
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - JBC1793861 JBC1793861
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - NG1809747 NG1809747
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - NG1831934 NG1831934
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - NG1840150 NG1840150
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - NG1914024 NG1914024
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 - NRG1713073 NRG1713073
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 -2521444 2521444
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 -2557579 2557579
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 -2561093 2561093
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 -2610396 2610396
      Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7), clone 4E12 -2654207 2654207
      Anti-RNA polymerase II subunit B1 -2724428 2724428
      Anti-RNA polymerase II subunit B1 -2768434 2768434

      References

      Reference overviewPub Med ID
      Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs.
      Brookes, Emily, et al.
      Cell Stem Cell, 10: 157-70 (2012) 2012

      Show Abstract
      22305566 22305566
      Cdc14 phosphatase promotes segregation of telomeres through repression of RNA polymerase II transcription.
      Clemente-Blanco, Andres, et al.
      Nat. Cell Biol., 13: 1450-6 (2011) 2011

      Show Abstract
      22020438 22020438

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies