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MABE878 | Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, ICC R Purified Monoclonal Antibody
      Catalogue NumberMABE878
      DescriptionAnti-RNA Helicase A/Dhx9 Antibody, clone 8E3
      Alternate Names
      • ATP-dependent RNA helicase A
      • RHA
      • DEAH box protein 9
      • mHEL-5
      • Nuclear DNA helicase II
      • NDH II
      Background InformationRNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3' to 5' direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment.
      Product Information
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
      Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dhx9
      • Ddx9
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~140 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA Helicase A/Dhx9, clone 8E3 - Q2367874 Q2367874
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2790047 2790047
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2791165 2791165
      Anti-RNA Helicase A/Dhx9, clone 8E3 -2821204 2821204


      Reference overviewPub Med ID
      Monoclonal antibody specific for Dhx9/NDHII/RHA.
      Kotani, Manato, et al.
      Hybridoma (Larchmt), 29: 259-61 (2010) 2010

      Show Abstract
      20569003 20569003