A part of MilliporeSigma

09-094 | Anti-RFC1 Antibody

09-094
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number09-094
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RFC1 Antibody
      Alternate Names
      • Replication Factor C1
      • RF-C
      • MHC binding factor, beta
      • A1
      • MGC51786
      • MHCBFB
      • PO-GA
      • RECC1
      • RFC
      • RFC140
      Background InformationReplication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability.
      References
      Product Information
      FormatPurified
      Presentation100 μg of rabbit polyclonal IgG in 100μl of 1X PBS with 0.1% sodium azide.
      Applications
      ApplicationAnti-RFC1 Antibody detects level of RFC1 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesSuggested dilutions:
      Western Blot: 1:1000
      Biological Information
      ImmunogenA synthetic peptide corresponding to amino acids 1125-1140 of human RFC1.
      HostRabbit
      SpecificityRFC1
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability.
      Gene Symbol
      • RFC1
      • RECC1
      • RFC140
      • PO-GA
      • A1
      • MHCBFB
      • MGC51786
      • RFC
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35251 # Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
      SIZE: 1148 amino acids; 128255 Da
      SUBUNIT: Heteropentamer of subunits of 140/145, 40, 38, 37, and 36.5 kDa that forms a complex with PCNA in the presence of ATP.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Wide tissue distribution. Undetectable in placental tissue.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P35251 ## Belongs to the activator 1 140 kDa subunit family. & Contains 1 BRCT domain.
      Molecular Weight145 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RFC1 (Rabbit polyclonal IgG) - DAM1411354 DAM1411354
      Anti-RFC1 - DAM1487583 DAM1487583
      Anti-RFC1 -2548048 2548048

      References

      Reference overviewPub Med ID
      1H, 15N and 13C resonance assignments of the BRCT region of the large subunit of human Replication Factor C
      Kobayashi, Masakazu and Siegal, Gregg
      J Biomol NMR, 31:183-4 (2005) 2005

      15772763 15772763
      Regulation of RelA (p65) function by the large subunit of replication factor C
      Anderson, Lisa A and Perkins, Neil D
      Mol Cell Biol, 23:721-32 (2003) 2003

      12509469 12509469
      Cell cycle-dependent phosphorylation of the large subunit of replication factor C (RF-C) leads to its dissociation from the RF-C complex
      Munshi, Anil, et al
      J Biol Chem, 278:48467-73 (2003) 2003

      12947101 12947101
      The large subunit of replication factor C interacts with the histone deacetylase, HDAC1
      Anderson, Lisa A and Perkins, Neil D
      J Biol Chem, 277:29550-4 (2002) 2002

      12045192 12045192