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ABC909 | Anti-Pyruvate Kinase (muscle) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, IHC, ICC Rb Purified Polyclonal Antibody
      Catalogue NumberABC909
      DescriptionAnti-Pyruvate Kinase (muscle) Antibody
      Alternate Names
      • Pyruvate kinase PKM
      • Pyruvate Kinase
      Background InformationPyruvate kinases are cellular glycolytic enzymes that mediate glucose metabolism. There are four mammalian isoforms encoded by two gnens. The L gene is alternatively spliced to produce the L (liver) and R (erythrocyte) isoforms, while the M (muscle) gene is alternatively spliced to produce the M1 or M2 isoform. Pyruvate kinase M1 (PKM1) is predominantly expressed in adult tissues, while PKM2 is expressed during embryonic development. Tumor cells express exclusively PKM2 and PKM2 knockdown significantly impairs cancer growth.
      Product Information
      PresentationPurified rabbit polyclonal 1XPBS, 20% Glycerol (pH7), and 0.025% proclin.
      ApplicationAnti-Pyruvate Kinase (muscle) Antibody detects level of Pyruvate Kinase and has been published and validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis (IHP): A 1:500 dilution from a representative lot detected Pyruvate Kinase in human lung carcinoma tissue.

      Immunocytochemistry Analysis (ICC): A 1:500 dilution from a representative lot detected Pyruvate Kinase in HeLa cells.
      Biological Information
      ImmunogenRecombinant fragment corresponding to human Pyruvate Kinase.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • PKM
      • OIP3
      • PK2
      • PK3
      • PKM2
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain, rat brain, HEK293, A431, HeLa and HepG2 lysates.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Pyruvate Kinase in mouse brain, rat brain, HEK293, A431, HeLa and HepG2 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies