A part of MilliporeSigma

MABE341-AF488 | Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC R AlexaFluor®488 Monoclonal Antibody
      Catalogue NumberMABE341-AF488
      DescriptionAnti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      This product is a conjugated version of Cat. No. MABE341.
      Product Information
      PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
      ApplicationThis Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
      Concentration0.5 mg/mL
      SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightRefer to Cat. No. MABE341 for observed molecular weight information.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information