AB5898 Anti-Protein Gene Product 9.5 Antibody

AB5898
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5898
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Protein Gene Product 9.5 Antibody
      Alternate Names
      • PGP 9.5
      • UCH-L1
      References
      Product Information
      FormatSerum
      Control
      • Brain tissue
      PresentationLiquid. Contains 0.05% sodium azide.
      Applications
      ApplicationAnti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:500.

      Optimal working dilutions must be determined by end user.

      blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
      Biological Information
      ImmunogenA 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityRecognizes Protein Gene Product 9.5 (PGP9.5).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryUCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
      Gene Symbol
      • UCHL1
      • PARK5
      • UCH-L1
      • Uch-L1
      • PGP9.5
      • EC 3.4.19.12
      • EC 6.-.-.-
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
      SIZE: 223 amino acids; 24824 Da
      SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary.
      PTM: O-glycosylated (By similarity).
      DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.
      SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family.
      MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5)2465127
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) - 31920403192040
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) -28880052888005
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY3008454
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY2925264
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY3143280
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY - 21200202120020
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY - 23032012303201
      GUINEA PIG ANTI-PROTEIN GENE PRODUCT 9.5 (PGP9.5) POLYCLONAL ANTIBODY - 21863172186317

      References

      Reference overviewApplicationSpeciesPub Med ID
      Changes in the Distribution of Periodontal Nerve Fibers during Dentition Transition in the Cat.
      Miki, K; Honma, S; Ebara, S; Kumamoto, K; Murakami, S; Wakisaka, S
      PloS one  10  e0129826  2015

      Show Abstract
      26083023 26083023
      Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization.
      Sibaev, A; Fichna, J; Saur, D; Yuece, B; Timmermans, JP; Storr, M
      World journal of gastrointestinal pharmacology and therapeutics  6  73-83  2015

      Show Abstract
      26261735 26261735
      Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control.
      Lin, LH; Moore, SA; Jones, SY; McGlashon, J; Talman, WT
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  18608-17  2013

      Show Abstract
      ImmunohistochemistryRat24259582 24259582
      Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice.
      Ataka, K; Asakawa, A; Nagaishi, K; Kaimoto, K; Sawada, A; Hayakawa, Y; Tatezawa, R; Inui, A; Fujimiya, M
      PloS one  8  e81744  2013

      Show Abstract
      24303068 24303068
      Enhanced insulin clearance in mice lacking TRPM8 channels.
      McCoy, DD; Zhou, L; Nguyen, AK; Watts, AG; Donovan, CM; McKemy, DD
      American journal of physiology. Endocrinology and metabolism  305  E78-88  2013

      Show Abstract
      23651844 23651844
      Characterization of behavioral and neuromuscular junction phenotypes in a novel allelic series of SMA mouse models.
      Osborne, M; Gomez, D; Feng, Z; McEwen, C; Beltran, J; Cirillo, K; El-Khodor, B; Lin, MY; Li, Y; Knowlton, WM; McKemy, DD; Bogdanik, L; Butts-Dehm, K; Martens, K; Davis, C; Doty, R; Wardwell, K; Ghavami, A; Kobayashi, D; Ko, CP; Ramboz, S; Lutz, C
      Human molecular genetics  21  4431-47  2012

      Show Abstract
      ImmunohistochemistryMouse22802075 22802075
      Chronic alcohol consumption induces an overproduction of NO by nNOS- and iNOS-expressing myenteric neurons in the murine small intestine.
      Bagyánszki M, Torfs P, Krecsmarik M, Fekete E, Adriaensen D, Van Nassauw L, Timmermans JP, Kroese AB.
      Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  23  e237-48  2011

      Show Abstract
      21470341 21470341
      The effect of inflammation on the expression and distribution of the MAS-related gene receptors MrgE and MrgF in the murine ileum.
      Avula LR, Buckinx R, Alpaerts K, Costagliola A, Adriaensen D, Van Nassauw L, Timmermans JP.
      Histochemistry and cell biology  136  569-85  2011

      Show Abstract
      21912971 21912971
      Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E.
      Kapeller, J; Möller, D; Lasitschka, F; Autschbach, F; Hovius, R; Rappold, G; Brüss, M; Gershon, MD; Niesler, B
      The Journal of comparative neurology  519  420-32  2011

      Show Abstract Full Text Article
      21192076 21192076
      Serotonin-immunoreactive neurons and mast cells in the mouse esophagus suggest involvement of serotonin in both motility control and neuroimmune interactions.
      Hempfling C, Neuhuber WL, Wörl J
      Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  2011

      Show Abstract
      22029710 22029710

      Data Sheet

      Title
      Anti-Protein Gene Product 9.5 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies