AB3786 Anti-Prosurfactant Protein C (proSP-C) Antibody

AB3786
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ELISA, IHC, IH(P), WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB3786
      Replaces07-647
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Prosurfactant Protein C (proSP-C) Antibody
      Alternate Names
      • proSP-C
      Background InformationPulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.
      References
      Product Information
      FormatSerum
      Control
      • Mouse lung epithelial cells.
      PresentationPolyclonal rabbit serum containing 0.01% sodium azide as a preservative.
      Applications
      ApplicationAnti-Prosurfactant Protein C (proSP-C) Antibody detects level of Prosurfactant Protein C (proSP-C) and has been published & validated for use in ELISA, Immunohistochemistry (IHC), Immunohistochemistry - Paraffin (IHC-P) and Western Blotting (WB).
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:2,000-1:4,000 dilution of a previous lot was used on human tissue (see protocol), 1:4,000 dilution on adult mouse lung and 1:1,000-1:2,000 dilution on fetal mouse lung (see protocol).
      Immunohistochemistry: 1:2,000-1:4,000 on human tissue with and without antigen retrieval (see protocol) and 1:1,000-1:2,000 on adult and 1:500-1:1,000 on fetal mouse lung (see notes).

      Immunoblot:
      1:5,000 (best if not reduced)

      Western Blot Analysis:
      a previous lot used a dilution of 1:500

      ELISA:
      1:50,000

      IMMUNOHISTOCHEMISTRY :
      Notes:
      1) Dilutions used are for normal (i.e. uninjured) adult mouse lung and have been used on FVB/N, Balb/c, nu/nu and SCID mice. The use of antigen retrieval systems to enhance immunostaining for this antibody is not necessary in the mouse lung and may actually increase background staining.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenA synthetic peptide corresponding to amino acids 1-32 from the N-terminal of the human SP-C proprotein coupled to KLH
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse and rat. Expected to cross-react with human.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark and Clark, 2005 [PubMed 15927881]). See also SFTPA1 (MIM 178630), SFTPB (MIM 178640), and SFTPD (MIM 178635).[supplied by OMIM] ...
      Gene Symbol
      • SFTPC
      • SP-C
      • SFTP2
      • SMDP2
      • Val.
      • SP5
      • PSP-C
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11686 # Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces.
      SIZE: 197 amino acids; 21053 Da
      SUBCELLULAR LOCATION: Secreted, extracellular space.
      DISEASE: SwissProt: P11686 # Polymorphisms in the SFTPC gene are important determinants for predisposition to respiratory distress syndrome (RDS) in premature infants [MIM:267450]. RDS in the newborn is the main cause of mortality and morbidity in premature infants. RDS is characterized by deficient gas exchange that is caused by diffuse atelectasis and high-permeability lung edema that results in fibrin-rich alveolar deposits called 'hyaline membranes'. The risk of bronchopulmonary dysplasia increases with the severity of RDS. & Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. SMDP2 is a rare autosomal dominant disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course. The pathophysiology of the disorder is postulated to involve intracellular accumulation of a structurally defective SFTPC protein. Some patients who survive to adulthood manifest features of pulmonary fibrosis such as shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees in inflammation, fibrosis, or both on biopsy. Pulmonary surfactant metabolism disorders are genetically heterogeneous and associated with various pathologic entities, including pulmonary alveolar proteinosis (PAP), desquamative interstitial pneumonitis (DIP), or cellular non-specific interstitial pneumonitis (NSIP). & Genetic variations in SFTPC are associated with respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS in the newborn is the main cause of mortality and morbidity in premature infants. RDS is characterized by deficient gas exchange that is caused by diffuse atelectasis and high-permeability lung edema that results in fibrin-rich alveolar deposits called 'hyaline membranes'. The risk of bronchopulmonary dysplasia increases with the severity of RDS.
      SIMILARITY: SwissProt: P11686 ## Contains 1 BRICHOS domain.
      MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
      Molecular Weight21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat lung lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected Prosurfactant Protein C on 10 μg of rat lung lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Prosurfactant Protein C (proSP-C)3073288
      Anti-Prosurfactant Protein C (proSP-C)2464523
      Anti-Prosurfactant Protein C (proSP-C) - 21179892117989
      Anti-Prosurfactant Protein C (proSP-C) - 23733082373308
      Anti-Prosurfactant Protein C (proSP-C) - 24466982446698
      Anti-Prosurfactant Protein C (proSP-C) - 20180542018054
      Anti-Prosurfactant Protein C (proSP-C) - 20346862034686
      Anti-Prosurfactant Protein C (proSP-C) - 20533312053331
      Anti-Prosurfactant Protein C (proSP-C) - 20877212087721
      Anti-Prosurfactant Protein C (proSP-C) - 22071552207155

      References

      Reference overviewApplicationSpeciesPub Med ID
      Lineage-negative progenitors mobilize to regenerate lung epithelium after major injury.
      Vaughan, AE; Brumwell, AN; Xi, Y; Gotts, JE; Brownfield, DG; Treutlein, B; Tan, K; Tan, V; Liu, FC; Looney, MR; Matthay, MA; Rock, JR; Chapman, HA
      Nature  517  621-5  2015

      Show Abstract
      25533958 25533958
      Fibroblast Growth Factor 9 Regulation by MicroRNAs Controls Lung Development and Links DICER1 Loss to the Pathogenesis of Pleuropulmonary Blastoma.
      Yin, Y; Castro, AM; Hoekstra, M; Yan, TJ; Kanakamedala, AC; Dehner, LP; Hill, DA; Ornitz, DM
      PLoS genetics  11  e1005242  2015

      Show Abstract
      25978641 25978641
      Conditional overexpression of TGFβ1 promotes pulmonary inflammation, apoptosis and mortality via TGFβR2 in the developing mouse lung.
      Sureshbabu, A; Syed, MA; Boddupalli, CS; Dhodapkar, MV; Homer, RJ; Minoo, P; Bhandari, V
      Respiratory research  16  4  2015

      Show Abstract
      Immunohistochemistry25591994 25591994
      Bax deficiency extends the survival of Ku70 knockout mice that develop lung and heart diseases.
      Ngo, J; Matsuyama, M; Kim, C; Poventud-Fuentes, I; Bates, A; Siedlak, SL; Lee, HG; Doughman, YQ; Watanabe, M; Liner, A; Hoit, B; Voelkel, N; Gerson, S; Hasty, P; Matsuyama, S
      Cell death & disease  6  e1706  2015

      Show Abstract
      25811803 25811803
      Sevoflurane ameliorates intestinal ischemia-reperfusion-induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress.
      Luo, C; Yuan, D; Zhao, W; Chen, H; Luo, G; Su, G; Hei, Z
      Molecular medicine reports  12  1082-90  2015

      Show Abstract
      25815524 25815524
      Lung Cancer Onset in Wild Type Mice Following Bone Marrow Reconstitution with kras(v12) Cells.
      Belloni, E; Martin Padura, I; Gerbino, E; Orecchioni, S; Fusar Imperatore, F; Marighetti, P; Bertalot, G; Giuseppe Pelicci, P; Bertolini, F
      Scientific reports  5  13047  2015

      Show Abstract
      26267334 26267334
      Analysis of lung tumor initiation and progression in transgenic mice for Cre-inducible overexpression of Cul4A gene.
      Wang, Y; Xu, Z; Mao, JH; Hung, MS; Hsieh, D; Au, A; Jablons, DM; You, L
      Thoracic cancer  6  480-7  2015

      Show Abstract
      26273405 26273405
      Control of Francisella tularensis Intracellular Growth by Pulmonary Epithelial Cells.
      Maggio, S; Takeda, K; Stark, F; Meierovics, AI; Yabe, I; Cowley, SC
      PloS one  10  e0138565  2015

      Show Abstract
      26379269 26379269
      Analysis of a lung defect in autophagy-deficient mouse strains.
      Cheong, H; Wu, J; Gonzales, LK; Guttentag, SH; Thompson, CB; Lindsten, T
      Autophagy  10  45-56  2014

      Show Abstract
      Immunohistochemistry24275123 24275123
      Expression level and subcellular localization of heme oxygenase-1 modulates its cytoprotective properties in response to lung injury: a mouse model.
      Namba, F; Go, H; Murphy, JA; La, P; Yang, G; Sengupta, S; Fernando, AP; Yohannes, M; Biswas, C; Wehrli, SL; Dennery, PA
      PloS one  9  e90936  2014

      Show Abstract
      24599172 24599172

      Data Sheet

      Title
      Anti-Prosurfactant Protein C - Data Sheet

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