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AB3430 | Anti-Prosurfactant Protein B Antibody, reacts with both CT and NT

AB3430
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M ELISA, IHC, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB3430
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Prosurfactant Protein B Antibody, reacts with both CT and NT
      Alternate Names
      • proSP-B
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Liquid. Contains no preservative.
      Applications
      ApplicationDetect Prosurfactant Protein B using this Anti-Prosurfactant Protein B Antibody, reacts with both C-terminus & N-terminus validated for use in ELISA, IH & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry: 1:4,000-1:8,000 on human tissue with and without antigen retrieval (see protocol on back) and 1:1,000-1:2,000 on adult and fetal mouse lung (see notes on back).

      Immunoblot: 1:1,000-1:5,000; proprotein 42-46kDa.

      ELISA: 1:500-1:1,000

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenFull length recombinant peptide (381 a.a.) of the human SP-B (pre)proprotein.
      Epitopereacts with both C-terminus and N-terminus
      HostRabbit
      SpecificityReacts strongly with human and mouse proSP-B (detects both the N-terminal and C-terminal propeptides). Does not react with mature SP-B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM]
      Gene Symbol
      • SFTPB
      • SP-B
      • SFTP3
      • Phe
      • PSP-B
      • SFTB3
      • SMDP1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07988 # Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
      SIZE: 381 amino acids; 42117 Da
      SUBUNIT: Homodimer; disulfide-linked.
      SUBCELLULAR LOCATION: Secreted, extracellular space.
      DISEASE: SwissProt: P07988 # Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1) [MIM:265120]; also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. Inborn errors of pulmonary surfactant metabolism are genetically heterogeneous disorders resulting in severe respiratory insufficiency or failure in full-term neonates or infants. These disorders are associated with various pathologic entities, including pulmonary alveolar proteinosis (PAP), desquamative interstitial pneumonitis (DIP), or cellular non- specific interstitial pneumonitis (NSIP).
      SIMILARITY: SwissProt: P07988 ## Contains 1 saposin A-type domain. & Contains 3 saposin B-type domains.
      MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to twelve months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) POLYCLONAL ANTIBODY - 2120944 2120944
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) POLYCLONAL ANTIBODY - 2428524 2428524
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) POLYCLONAL ANTIBODY - 2446697 2446697
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) - 2470363 2470363
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2547769 2547769
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2615690 2615690
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2631283 2631283
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2685734 2685734
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2691402 2691402
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2695513 2695513
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2701555 2701555
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) -2820408 2820408
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) POLYCLONAL ANTIBODY - 2234102 2234102
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B) POLYCLONAL ANTIBODY - 2343131 2343131

      References

      Reference overviewApplicationSpeciesPub Med ID
      Alveolar epithelial differentiation of human induced pluripotent stem cells in a rotating bioreactor.
      Ghaedi, M; Mendez, JJ; Bove, PF; Sivarapatna, A; Raredon, MS; Niklason, LE
      Biomaterials 35 699-710 2014

      Show Abstract
      Immunocytochemistry24144903 24144903
      Gestational exposure of mice to secondhand cigarette smoke causes bronchopulmonary dysplasia blocked by the nicotinic receptor antagonist mecamylamine.
      Singh, SP; Gundavarapu, S; Smith, KR; Chand, HS; Saeed, AI; Mishra, NC; Hutt, J; Barrett, EG; Husain, M; Harrod, KS; Langley, RJ; Sopori, ML
      Environmental health perspectives 121 957-64 2013

      Show Abstract
      23757602 23757602
      Measurement of human surfactant protein-B turnover in vivo from tracheal aspirates using targeted proteomics.
      Tomazela, DM; Patterson, BW; Hanson, E; Spence, KL; Kanion, TB; Salinger, DH; Vicini, P; Barret, H; Heins, HB; Cole, FS; Hamvas, A; MacCoss, MJ
      Analytical chemistry 82 2561-7 2010

      Show Abstract
      20178338 20178338
      Developmental and genetic regulation of human surfactant protein B in vivo.
      Hamvas, A; Heins, HB; Guttentag, SH; Wegner, DJ; Trusgnich, MA; Bennet, KW; Yang, P; Carlson, CS; An, P; Cole, FS
      Neonatology 95 117-24 2009

      Show Abstract Full Text Article
      18776725 18776725
      Increased epithelial cell proliferation in very premature baboons with chronic lung disease.
      William M Maniscalco, Richard H Watkins,
      American journal of physiology. Lung cellular and molecular physiology 283 L991-L1001 2002

      Show Abstract
      12376352 12376352
      Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency.
      Nogee, L M, et al.
      Am. J. Respir. Crit. Care Med., 161: 973-81 (2000) 2000

      Show Abstract
      Immunoblotting (Western)Human10712351 10712351
      Congenital cystic adenomatoid malformation of the lung (CCAM): evaluation of the cellular components.
      Morotti, R A, et al.
      Hum. Pathol., 30: 618-25 (1999) 1999

      Show Abstract
      10374767 10374767

      Data Sheet

      Title
      RABBIT ANTI-HUMAN PROSURFACTANT PROTEIN B (proSP-B)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies