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MABC953 | Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Mk WB, ICC, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABC953
      DescriptionAnti-Prohibitin-2/PHB2 Antibody, clone 7F8E3
      Alternate Names
      • Prohibitin-2
      • B-cell receptor-associated protein BAP37
      • D-prohibitin
      • Repressor of estrogen receptor activity
      • Prohibitin-2/PHB2
      Background InformationProhibitin-2 (UniProt Q99623; also known as B-cell associated protein, B-cell receptor-associated protein BAP37, BCAP37, D-prohibitin, PHB2, Repressor of estrogen receptor activity) is encoded by the PHB2 (also known as BAP, REA) gene (Gene ID 11331) in human. Prohibitins are assembled into a ring-like PHB complex with 16-20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane, where they function as chaperone for respiration chain proteins or as important structural scaffold for optimal mitochondrial morphology and function. Prohibitins are also reported to modulate transcriptions by interacting with various transcription factors, including nuclear receptors. Upregulated expression of prohibitins, septin 9, neurabin 1, and other cytoskeletal and functional proteins is found at the glutathione S-transferase placental-form-positive (GST-P+) hepatocellular carcinoma (HCC) foci during initiation and/or promotion stages of rat hepatocarcinogenesis, implicating an involvement of prohibitins in the neoplastic transformation of rat liver preneoplastic lesions.
      Product Information
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of Prohibitin-2/PHB2.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Prohibitin-2/PHB2 in 10 µg of COS-7 cell lysate.
      Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue.
      Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Prohibitin-2/PHB2.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with both spliced isoforms.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PHB2
      • BAP
      • REA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~34 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat C6 glioma cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Prohibitin-2/PHB2, clone 7F8E3 -2691478 2691478
      Anti-Prohibitin-2/PHB2, clone 7F8E3 -Q2592801 Q2592801


      Reference overviewPub Med ID
      Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.
      Kakehashi, A; Ishii, N; Shibata, T; Wei, M; Okazaki, E; Tachibana, T; Fukushima, S; Wanibuchi, H
      Toxicological sciences : an official journal of the Society of Toxicology 119 61-72 2011

      Show Abstract
      20935162 20935162

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

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      Life Science Research > Antibodies and Assays > Primary Antibodies