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MABN1795 | Anti-Progranulin/GRN Antibody, clone 8H10

100 μg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB, IHC R Purified Monoclonal Antibody
      Catalogue NumberMABN1795
      DescriptionAnti-Progranulin/GRN Antibody, clone 8H10
      Alternate Names
      • Granulins
      • Progranulin/GRN
      Background InformationGranulins constitute a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 602 aa murine precursor protein, preprogranulin (UniProt Q3U9N4), is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide (aa 1-17) produces progranulin (Acrogranin; aa 18-589), which can be further cleaved into 7 active peptides named granulin-1 through granulin-7. Both the peptides and progranulin protein regulate cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Heterozygous loss-of-function mutations in the GRN gene is a known cause for frontotemporal lobar degeneration (FTLD) with accumulation of TAR DNA-binding protein (TDP)-43, while a complete GRN deficiency due to a homozygous loss-of-function mutation causes the lysosomal storage disorder known as neuronal ceroid lipofuscinosis (NCL). In Grn-/- mice, a murine model for GRN-associated FTLD-TDP (FTLD-TDP/GRN), the lysosomal proteins cathepsin D (CTSD) is found to be elevated, while an upregulated granulin level is observed in Ctsd(−/−) mice, a model for NCL. Moreover, both types of knockout mice are found to display increased levels of transmembrane protein 106B (TMEM106B) as a common phenotype.
      Product Information
      PresentationPurified rat monoclonal IgG2cκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-Progranulin/GRN Antibody, clone 8H10 is validated for use in Western Blotting, Immunohistochemistry for the detection of Progranulin/GRN.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected elevated progranulin expression in brain homogenates from Ctsd-/- mice, and loss of progranulin expression in brain homogenates from Grn-/- mice (Gotzl, J.K., et al (2014). Acta Neuropathol.127(6):845-860).
      Immunohistochemistry Analysis: A representative lot detected elevated progranulin expression in the cortex, hippocampus, and thalamus regions of Ctsd-/- mice when compared with wild-type mice using frozen brain sections (Gotzl, J.K., et al (2014). Acta Neuropathol.127(6):845-860).
      Biological Information
      ImmunogenLinear peptide corresponding to rat progranulin sequence near the C-terminus
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityReacts with Preprogranulin and Progranulin (Acrogranin, Proepithelin), but not Granulin-1 through Granulin-7.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Grn
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse uterus tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Progranulin/GRN in 10 µg of mouse uterus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Progranulin/GRN, clone 8H10 -Q2592790 Q2592790


      Reference overviewPub Med ID
      Common pathobiochemical hallmarks of progranulin-associated frontotemporal lobar degeneration and neuronal ceroid lipofuscinosis.
      Götzl, JK; Mori, K; Damme, M; Fellerer, K; Tahirovic, S; Kleinberger, G; Janssens, J; van der Zee, J; Lang, CM; Kremmer, E; Martin, JJ; Engelborghs, S; Kretzschmar, HA; Arzberger, T; Van Broeckhoven, C; Haass, C; Capell, A
      Acta neuropathologica 127 845-60 2014

      Show Abstract
      24619111 24619111

      Technical Info

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